Document Detail


Phospholipid biosynthesis in the anaerobic protozoon Entodinium caudatum.
MedLine Citation:
PMID:  808216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The anaerobic rumen protozoon Entodinium caudatum was incubated either intact or with various radioactive precursors of phospholipids after ultrasonication. 2. Pulse-chase experiments showed a rapid turnover of phosphatidylinositol and much slower turnovers of phosphatidylethanolamine and phosphatidylcholine. 3. E. caudatum imbibed choline very rapidly; this was immediately and exclusively converted into phosphatidylcholine which was shown by radioautography after 10 min to be distributed throughout the cell membranes. 4. Phosphatidylcholine was synthesized through a phosphorylcholine-CDP-choline pathway, the methylation or base-exchange pathways not being present. 5. Under suitable conditions [Me-14C]choline can be substantially (50-60%) converted into CDP-choline by sonicated E. caudatum and this provides an excellent method of preparing this biosynthetic intermediary. 6. [2-14C]Ethanolamine was taken up much less readily than choline. The former was incorporated into phosphatidylethanolamine by the CDP-ethanolamine pathway. 7. Doubly labelled [32P]phosphatidyl[2-3H]ethanolamine was converted into ceramide phosphorylethanolamine and N-(1-carboxyethyl)phosphatidyl-ethanolamine, without change in the isotopic ratio. Ceramide phosphoryl [2-14C]-ethanolamine was converted into phsophatidylethanolamine. 8. Palmitic acid, oleic acid and linoleic acid were taken by E. caudatum cells and incorporated into phospholipids. By contrast, although stearic acid was taken up it was hardly incorporated into phospholipids.
Authors:
T E Broad; R M Dawson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  146     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-11-07     Completed Date:  1975-11-07     Revised Date:  2010-09-03    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  317-28     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Autoradiography
Ceramides / biosynthesis
Choline / metabolism
Ciliophora / metabolism*
Cytidine Diphosphate Choline / biosynthesis
Cytosine Nucleotides / metabolism
Ethanolamines / metabolism
Fatty Acids / metabolism
Methionine / metabolism
Phosphatidylethanolamines / metabolism
Phosphatidylinositols / metabolism
Phospholipids / biosynthesis*
Serine / metabolism
Sonication
Chemical
Reg. No./Substance:
0/Ceramides; 0/Cytosine Nucleotides; 0/Ethanolamines; 0/Fatty Acids; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phospholipids; 56-45-1/Serine; 62-49-7/Choline; 63-68-3/Methionine; 987-78-0/Cytidine Diphosphate Choline
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