Document Detail


Phospholipid accumulation during the cell cycle in synchronous cultures of the yeast, Saccharomyces cerevisiae.
MedLine Citation:
PMID:  6270139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phospholipid concentrations have been examined throughout successive cell cycles in synchronously growing cultures of the yeast, Saccharomyces cerevisiae. Total phospholipid phosphorus, as well as lecithin and phosphatidylethanolamine levels, exhibited stepwise increases during the cell cycle with step increments beginning just prior to new rounds of bud formation. Phosphatidylinositol and phosphatidylserine levels, on the other hand, showed what have been interpreted to be peak concentrations near the time of bud formation. Cardiolipin content varied considerably and was dependent upon the carbon source of the growth medium. Glucose-grown cells exhibited peak concentrations of cardiolipin near the time of bud formation, with marked decreases after this time. In contrast, galactose-grown synchronous cells exhibited stepwise increments in cardiolipin content, with step increases occurring near the time of new rounds of bud formation. Step or peak increases in cardiolipin, as well as all other phospholipids, were found to coincide with the time of stepwise increases in cytochrome c oxidase activity in these cells. No correlations were observed between the elaboration of mitochondrial membranes during the synchronous cell cycle and the observed patterns of phospholipid increase.
Authors:
S F Cottrell; G S Getz; M Rabinowitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10973-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cardiolipins / metabolism
Cell Cycle
DNA, Fungal / metabolism
Electron Transport Complex IV / metabolism
Glucose / metabolism
Intracellular Membranes / ultrastructure
Kinetics
Membrane Lipids / metabolism
Mitochondria / metabolism,  ultrastructure
Phospholipids / metabolism*
Saccharomyces cerevisiae / physiology*
Grant Support
ID/Acronym/Agency:
GM 18858/GM/NIGMS NIH HHS; GM 25901/GM/NIGMS NIH HHS; HL 04442/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cardiolipins; 0/DNA, Fungal; 0/Membrane Lipids; 0/Phospholipids; 50-99-7/Glucose; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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