| Phospholipid Peroxidation: Lack of Effect of Fatty Acid Pairing. | |
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MedLine Citation:
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PMID: 22271423 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Phospholipids where both fatty acids are polyunsaturated are very rare. Most organisms prefer to couple their polyunsaturated fatty acids (PUFA) with either a saturated (SAT) or a monounsaturated (MUFA) fatty acid. This study examined if these natural couplings are there to protect PUFA from themselves. Specifically, does the coupling of PUFA to SAT or MUFA reduce the potential for increased rates of peroxidation by shrouding these highly peroxidisable fatty acids with less peroxidisable fatty acids? The influence of head group was examined by using the two most common phospholipids found in vertebrate membranes i.e. phosphatidylcholine and phosphatidylethanolamine species. Fatty acid pairings included 16:0/18:2 versus 18:2/18:2 and 16:0/22:6 versus 22:6/22:6. All phospholipids were incorporated into liposomes that were matched for their total PUFA content i.e. 25% PUFA/PUFA or 50% SAT/PUFA with phosphatidylcholine 16:0/16:0 used as the background phospholipid. An iron initiator (Fe(2+)/H(2)O(2)) was used to induce peroxidation and lipid hydroperoxide production was used to measure peroxidation. The results show that coupling of PUFA together on the same molecule does not increase peroxidation rates and therefore does not support the proposed hypothesis. The lower than expected levels of peroxidation measured for some phospholipid species (e.g. PtdEtn 22:6/22:6) is possibly due to the partitioning of these molecular species into the inner leaflet of the bilayer. |
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Authors:
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Sarah E Norris; Todd W Mitchell; Paul L Else |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-24 |
Journal Detail:
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Title: Lipids Volume: - ISSN: 1558-9307 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Metabolic Research Centre, School of Health Sciences, Illawarra Health and Medical Research Institute (IHMRI), University of Wollongong, Wollongong, NSW, 2522, Australia, sen965@uowmail.edu.au. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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