Document Detail

Phospholipid Fatty Acid Composition of the Syntrophic Anaerobic Bacterium Syntrophomonas wolfei.
MedLine Citation:
PMID:  16347667     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The membrane phospholipid fatty acids (PLFAs) from several cocultures and a pure culture of Syntrophomonas wolfei were determined by capillary column gas chromatography. Cocultures of S. wolfei with a Desulfovibrio sp. contained PLFAs from both organisms, whereas PLFAs from a coculture with Methanospirillum hungatei contained very little biomass to analyze. The pure culture of S. wolfei grown on crotonate provided the best material for analysis of the PLFAs. The predominant PLFAs of S. wolfei were the monounsaturated 16:1omega7c and 16:1omega9c and the saturated 16:0 and 14:0. A low concentration of the diunsaturated 18:2omega6 was detected. The PLFA analysis provides additional information for consideration in the determination of the profile of PLFAs obtained from anaerobic environments. In addition, this information may aid in the understanding of the physiology and phylogeny of S. wolfei and other syntrophic bacteria.
J M Henson; M J McInerney; P S Beaty; J Nickels; D C White
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  54     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-07-02     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1570-4     Citation Subset:  -    
Agricultural Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Monticello, Florida 32344; Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019 ; and Department of Biological Sciences, Florida State University, Tallahassee, Florida 32306.
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