| Phospholipases A2 in ras-transfected fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 8572584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phospholipase A2 (PLA2) generated lipid biomediators can facilitate neoplastic progression. Specific PLA2 alterations associated with ras oncogene expression were determined by comparison of PLA2 activities in nontumorigenic rat embryo fibroblasts (CREF cells) and their tumorigenic ras oncogene-transfected counterparts (CREF-T24 cells). The high molecular mass cytosolic PLA2 activity is 2-3 fold higher in CREF-T24 cells as compared to CREF cells. Western blotting analyses indicate increases in the level of this enzyme and the proportion which migrates with phosphorylated enzyme in CREF-T24 cells. A PLA2 activity, with the characteristics of a group II PLA2, is readily detectible in particulate fractions from CREF-T24 cells following ammonium sulfate extraction/cation ion exchange chromatography. In contrast, this activity is minimal in similarly prepared CREF cell samples. While the CREF-T24 cells have increases in two PLA2 activities associated with the release of arachidonic acid, the CREF-T24 and CREF cells are similar with respect to Ca2+ independent, particulate fraction-associated PLA2 activities. |
| | |
Authors:
|
C J Guthridge; S G Zimmer; M R Steiner |
Related Documents
:
|
10464334 - Contact-dependent growth inhibition and apoptosis of epidermal growth factor (egf) rece... 11360924 - Shikonin, a component of antiinflammatory chinese herbal medicine, selectively blocks c... 15803204 - Transgene delivery and gelonin cytotoxicity enhanced by photochemical internalization i... 8564934 - Nuclear localization of procathepsin l/mep in ras-transformed mouse fibroblasts. 15327974 - Phosphate or phosphite addition promotes the proteolytic turnover of phosphate-starvati... 11112704 - Cdc28-clb mitotic kinase negatively regulates bud site assembly in the budding yeast. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Anticancer research Volume: 15 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 1995 Sep-Oct |
Date Detail:
|
Created Date: 1996-03-01 Completed Date: 1996-03-01 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
|
Languages: eng Pagination: 1957-62 Citation Subset: IM |
Affiliation:
|
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid / metabolism Cytokines / biosynthesis Fibroblasts / enzymology Genes, ras* Hela Cells Humans Phospholipases A / metabolism* Phospholipases A2 Rats Transfection |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 506-32-1/Arachidonic Acid; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chemoprevention of diethylnitrosamine-induced liver foci and hepatocellular adenomas in C3H mice.
Next Document: Hemin toxicity in a human epithelioid sarcoma cell line.