Document Detail

Phospholipase activity in rat liver mitochondria studied by the use of endogenous substrates.
MedLine Citation:
PMID:  5971043     Owner:  NLM     Status:  MEDLINE    
The hydrolysis of endogenous phosphatidyl ethanolamine and lecithin in rat liver mitochondria has been studied by using mitochondria from rats injected with ethanolamine-1,2-(14)C or choline-1,2-(14)C. A phospholipase A-like enzyme has been demonstrated, which catalyzes the hydrolysis of one fatty acid ester linkage in phosphatidyl ethanolamine and lecithin. Phosphatidyl ethanolamine is hydrolyzed in preference to lecithin and the main reaction products are free fatty acids and lysophosphatidyl ethanolamine. The further breakdown of lysophospholipids appears to be limited in mitochondria, which indicates that lysophospholipase activity is mainly located extramitochondrially. The enzymic system is greatly stimulated by calcium ions, and also slightly by magnesium ions, while EDTA inhibits it almost completely. These findings are discussed in relation to previous observations on the effect of calcium and of EDTA on the functions of mitochondria. The possible function of the mitochondrial phospholipase for the formation of phospholipids with special fatty acids at the alpha- and -position is discussed.
P Bjornstad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  7     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1966 Sep 
Date Detail:
Created Date:  1967-09-07     Completed Date:  1967-09-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  612-20     Citation Subset:  IM    
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MeSH Terms
Amino Alcohols / metabolism
Calcium / pharmacology
Carbon Isotopes
Choline / metabolism
Fatty Acids / metabolism
Hot Temperature
Hydrogen-Ion Concentration
Liver / enzymology
Magnesium / pharmacology
Mitochondria / enzymology*
Phosphatidylcholines / metabolism*
Phosphatidylethanolamines / metabolism*
Phospholipases / metabolism*
Reg. No./Substance:
0/Amino Alcohols; 0/Carbon Isotopes; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 62-49-7/Choline; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 3.1.-/Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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