| Phospholipase D modulation by ceramide in senescence. | |
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MedLine Citation:
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PMID: 19856145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipase D (PLD) has been implicated in mitotic regulation and has been shown to be defective in cells following replicative senescence. We examined the source of changes in PLD activity in senescent human umbilical vein endothelial cells and in human diploid fibroblasts. Using fractionated cell components we found that the cytosolic components were necessary for maximum PLD activation. In comparison to low-passage cells, senescent cells showed a profound lack of PLD activatability. By recombining fractionated components from senescent and low-passage cells, we found that in senescence the membrane component is defective in activating PLD implicating either the PLD enzyme itself or its interaction with PKC and/or ARF. The sphingolipid ceramide has been implicated in mediating senescence. Treatment with ceramide resulted in a decrease in PLD activity, implicating ceramide as the mediator of the inhibition. |
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Authors:
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Lisa M Webb; Alan T Arnholt; Mark E Venable |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-25 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 337 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-11 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 153-8 Citation Subset: IM |
Affiliation:
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Biology Department, Appalachian State University, Boone, NC 28608-2027, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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drug effects*,
physiology Cell Count Cell Extracts / pharmacology Cell Fractionation Cell Membrane / chemistry, physiology Cells, Cultured Ceramides / pharmacology* Cytosol / chemistry, physiology Dose-Response Relationship, Drug Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Humans Phospholipase D / antagonists & inhibitors, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Ceramides; 0/Enzyme Inhibitors; 0/N-caproylsphingosine; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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