Document Detail


Phospholipase D contributes to transmural pressure control of prorenin processing in juxtaglomerular cell.
MedLine Citation:
PMID:  11882574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to delineate the involvement of phospholipase C (PLC) and phospholipase D (PLD) in transmural pressure control of renin synthesis and secretion. Primary cultures of rat juxtaglomerular (JG) cells were applied to a transmural pressure-loading apparatus for 12 hours, and the renin secretion rate (RSR), active renin content (ARC), and total (active + inactive) renin content (TRC) were determined. Under control conditions (n=5), transmural pressure decreased RSR (78.1 +/- 3.0 and 64.6 +/- 4.4% for 0 or 40 mm Hg, respectively; P<0.05) and ARC (42.8 +/- 3.3 and 26.0 +/- 3.9 ng of angiotensin I per hour per million cells for 0 or 40 mm Hg, respectively; P<0.05) but did not have a significant effect on TRC (99.5 +/- 6.7 and 89.2 +/- 4.6 ng of angiotensin I per hour per million cells for 0 or 40 mm Hg, respectively). Treatment with PLC inhibitors, 2-nitro-4-carboxyphenyl-N,N-diphenyl-carbamate (200 micromol/L) and U73122 (10 micromol/L) did not alter RSR but prevented the RSR decrease with transmural pressure, whereas neither 2-nitro-4-carboxyphenyl-N,N-diphenyl-carbamate nor U73122 altered ARC, TRC, or the decrease in ARC with transmural pressure. Experiments were also performed using JG cells (n=5) treated with a PLD inhibitor, 4-(2-aminoethyl)-benzensulfonyl fluoride (AEBSF, 100 micromol/L). Treatment with AEBSF did not influence basal levels of RSR, ARC, and TRC or the RSR decrease with transmural pressure. However, AEBSF did inhibit the decrease in ARC with transmural pressure. These results indicate that transmural pressure inhibits renin secretion via PLC-dependent pathways and prevents conversion of inactive renin to active renin via PLD-dependent mechanisms in JG cells.
Authors:
Nobuhisa Hirota; Atsuhiro Ichihara; Yukako Koura; Matsuhiko Hayashi; Takao Saruta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hypertension     Volume:  39     ISSN:  1524-4563     ISO Abbreviation:  Hypertension     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-07     Completed Date:  2002-03-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-7     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antithrombins / pharmacology
Cells, Cultured
Enzyme Precursors / metabolism*
Estrenes / pharmacology
Juxtaglomerular Apparatus / cytology*,  drug effects
Male
Phospholipase D / metabolism*
Pyrrolidinones / pharmacology
Rats
Rats, Sprague-Dawley
Renin / metabolism*
Renin-Angiotensin System / physiology
Sulfones / pharmacology
Type C Phospholipases / metabolism
Chemical
Reg. No./Substance:
0/Antithrombins; 0/Enzyme Precursors; 0/Estrenes; 0/Pyrrolidinones; 0/Sulfones; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 142878-12-4/U 73343; 34284-75-8/4-(2-aminoethyl)benzenesulfonylfluoride; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.4/Phospholipase D; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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