| Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice. | |
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MedLine Citation:
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PMID: 19056831 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lymphatic vasculature originates from the blood vasculature through a mechanism relying on Prox1 expression and VEGFC signalling, and is separated and kept separate from the blood vasculature in a Syk- and SLP76-dependent manner. However, the mechanism by which lymphatic vessels are separated from blood vessels is not known. To gain an understanding of the vascular partitioning, we searched for the affected gene in a spontaneous mouse mutant exhibiting blood-filled lymphatic vessels, and identified a null mutation of the Plcg2 gene, which encodes phospholipase Cgamma2 (PLCgamma2), by positional candidate cloning. The blood-lymph shunt observed in PLCgamma2-null mice was due to aberrant separation of blood and lymphatic vessels. A similar phenotype was observed in lethally irradiated wild-type mice reconstituted with PLCgamma2-null bone marrow cells. These findings indicate that PLCgamma2 plays an essential role in initiating and maintaining the separation of the blood and lymphatic vasculature. |
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Authors:
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Hirotake Ichise; Taeko Ichise; Osamu Ohtani; Nobuaki Yoshida |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-04 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 136 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-23 Completed Date: 2009-03-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 191-5 Citation Subset: IM |
Affiliation:
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Laboratory of Gene Expression and Regulation, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan. h-ichise@ims.u-tokyo.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blood Vessels / abnormalities, embryology*, enzymology* Bone Marrow Transplantation Cells, Cultured DNA Primers / genetics Endothelial Cells / enzymology Female Humans Lymphatic Vessels / abnormalities, embryology*, enzymology* Mice Mice, Knockout Mice, Mutant Strains Mice, Transgenic Phospholipase C gamma / deficiency, genetics, physiology* Polymorphism, Genetic Pregnancy Radiation Chimera |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 3.1.4.3/Phospholipase C gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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