Document Detail


Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction.
MedLine Citation:
PMID:  18710717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the localization of phospholipase C zeta (PLC zeta) in non-capacitated, capacitated, and ionophore-treated sperm.
DESIGN: Phospholipase C zeta was cloned from the hamster, an important model organism for studying fertilization. Next, we used hamster and mouse models to investigate the localization of PLC zeta in non-capacitated and capacitated sperm and in sperm treated with ionophore to induce the acrosome reaction.
SETTING: University laboratory.
ANIMAL(S): Male mice and hamsters, 4-6 weeks old.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Phospholipase C zeta localization in non-capacitated, capacitated, and ionophore-treated sperm.
RESULT(S): Full-length hamster PLC zeta complementary DNA is 1953 base pairs in size, encoding an open reading frame of 651 amino acids, sharing 85% amino acid similarity with the mouse. Phospholipase C zeta was localized in acrosomal and post-acrosomal regions of sperm. The post-acrosomal localization, which became more evident after capacitation and was maintained after ionophore treatment, is in line with PLC zeta being the endogenous agent of egg activation. However, the acrosomal PLC zeta population, which was lost after ionophore treatment, suggests that PLC zeta could have other functions besides egg activation.
CONCLUSION(S): Phospholipase C zeta is localized to acrosomal and post-acrosomal regions and undergoes dynamic changes during capacitation and the acrosome reaction, indicating a potential role regulating not only egg activation but other sperm functions.
Authors:
Claire Young; Patricia Grasa; Kevin Coward; Lianne C Davis; John Parrington
Related Documents :
9022757 - Alterations of fatty acyl turnover in macrophage glycerolipids induced by stimulation. ...
18176897 - Alpha-synuclein decreases arachidonic acid incorporation into rat striatal synaptoneuro...
12170587 - Phospholipid composition and [14c]glycerol incorporation into glycerolipids of toad ret...
6148917 - Enhanced de novo synthesis of phosphatidic acid and phosphatidylinositol in rat pancrea...
15940877 - Changes in content and fatty acid profiles of total lipids and sulfolipids in the halop...
23340797 - The first dehydration and the competing reaction pathways of glucose homogeneously and ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-16
Journal Detail:
Title:  Fertility and sterility     Volume:  91     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-05-29     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2230-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acrosome / enzymology
Acrosome Reaction / physiology*
Animals
Blotting, Northern
Cloning, Molecular
Cricetinae
DNA Primers
Immunoblotting
Male
Mesocricetus
Mice
Mice, Inbred Strains
Phosphoinositide Phospholipase C / genetics*,  metabolism
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sperm Capacitation / physiology*
Spermatozoa / enzymology*
Grant Support
ID/Acronym/Agency:
G0500672//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Recombinant Proteins; EC 3.1.4.11/Phosphoinositide Phospholipase C; EC 3.1.4.3/Plcz1 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Persistence of androgenic effects on the production of proinflammatory cytokines by circulating anti...
Next Document:  Bacterial endotoxin (LPS)-induced DNA damage in preimplanting embryonic and uterine cells inhibits i...