Document Detail

Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction.
MedLine Citation:
PMID:  18710717     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the localization of phospholipase C zeta (PLC zeta) in non-capacitated, capacitated, and ionophore-treated sperm.
DESIGN: Phospholipase C zeta was cloned from the hamster, an important model organism for studying fertilization. Next, we used hamster and mouse models to investigate the localization of PLC zeta in non-capacitated and capacitated sperm and in sperm treated with ionophore to induce the acrosome reaction.
SETTING: University laboratory.
ANIMAL(S): Male mice and hamsters, 4-6 weeks old.
MAIN OUTCOME MEASURE(S): Phospholipase C zeta localization in non-capacitated, capacitated, and ionophore-treated sperm.
RESULT(S): Full-length hamster PLC zeta complementary DNA is 1953 base pairs in size, encoding an open reading frame of 651 amino acids, sharing 85% amino acid similarity with the mouse. Phospholipase C zeta was localized in acrosomal and post-acrosomal regions of sperm. The post-acrosomal localization, which became more evident after capacitation and was maintained after ionophore treatment, is in line with PLC zeta being the endogenous agent of egg activation. However, the acrosomal PLC zeta population, which was lost after ionophore treatment, suggests that PLC zeta could have other functions besides egg activation.
CONCLUSION(S): Phospholipase C zeta is localized to acrosomal and post-acrosomal regions and undergoes dynamic changes during capacitation and the acrosome reaction, indicating a potential role regulating not only egg activation but other sperm functions.
Claire Young; Patricia Grasa; Kevin Coward; Lianne C Davis; John Parrington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-16
Journal Detail:
Title:  Fertility and sterility     Volume:  91     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-05-29     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2230-42     Citation Subset:  IM    
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MeSH Terms
Acrosome / enzymology
Acrosome Reaction / physiology*
Blotting, Northern
Cloning, Molecular
DNA Primers
Mice, Inbred Strains
Phosphoinositide Phospholipase C / genetics*,  metabolism
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sperm Capacitation / physiology*
Spermatozoa / enzymology*
Grant Support
G0500672//Medical Research Council; //Medical Research Council
Reg. No./Substance:
0/DNA Primers; 0/Recombinant Proteins; EC Phospholipase C; EC protein, mouse

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