Document Detail

Phospholipase C mediated Ca2+ signals in murine urinary bladder smooth muscle.
MedLine Citation:
PMID:  19303404     Owner:  NLM     Status:  MEDLINE    
Muscarinic stimulation of urinary bladder induces contraction via an increase in intracellular Ca(2+) concentration that results from Ca(2+) influx through Ca(2+) channels and/or IP(3)-mediated Ca(2+) release controlled by phospholipase C (PLC) signalling. The significance of PLC/IP(3) signalling in this cascade has recently been questioned because PLC inhibitors were without effect on carbachol-induced contractions in detrusor muscle strips. However, PLC/IP(3)-mediated Ca(2+) release was clearly observed in recordings of Ca(2+) signals in isolated myocytes. Therefore, we investigated the presence of PLC/IP(3)-dependent Ca(2+) release by directly monitoring Ca(2+) signals in intact detrusor muscle strips. Concomitant Ca(2+) signals from Ca(2+) channel activity were eliminated by the Ca(2+) channel antagonist isradipine (3 microM) or by the use of muscles from Ca(v)1.2 channel-deficient (SMACKO) mice. In absence of Ca(2+) channel activity, carbachol elicited contractions and Ca(2+) signals in muscles from wild type and SMACKO mice that were inhibited by the PLC inhibitor U73122 (10 microM). The results show that PLC/IP(3)-dependent Ca(2+) release is activated by stimulation with carbachol in urinary bladder smooth muscle but has a minor contribution to overall carbachol-induced Ca(2+) signals.
Eva Frei; Franz Hofmann; Jörg W Wegener
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-19
Journal Detail:
Title:  European journal of pharmacology     Volume:  610     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  106-9     Citation Subset:  IM    
Institut für Pharmakologie und Toxikologie, TU München, Biedersteiner Str. 29, D-80802 München, Germany.
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MeSH Terms
Calcium / metabolism*
Calcium Channel Blockers / pharmacology
Carbachol / antagonists & inhibitors,  pharmacology
Cholinergic Agonists / pharmacology
Enzyme Inhibitors / pharmacology
Estrenes / pharmacology
Isradipine / pharmacology
Mice, Knockout
Muscle Contraction / drug effects
Muscle, Smooth / drug effects*,  physiology
Pyrrolidinones / pharmacology
Signal Transduction / drug effects*
Time Factors
Type C Phospholipases / metabolism*
Urinary Bladder / drug effects*,  metabolism
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Cholinergic Agonists; 0/Enzyme Inhibitors; 0/Estrenes; 0/Pyrrolidinones; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 51-83-2/Carbachol; 7440-70-2/Calcium; 75695-93-1/Isradipine; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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