Document Detail


Phospholipase C activity is enhanced in skin fibroblasts obtained from patients with essential hypertension.
MedLine Citation:
PMID:  12640253     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: In human hypertension, the response of phospholipase C (PLC) to stimuli is enhanced in signal transduction where receptors are coupled to pertussis toxin-sensitive G protein. We investigated PLC activity and its role in human hypertension. METHODS AND RESULTS: Skin fibroblasts were cultured from 15 normotensives subjects (53 +/- 4 years, four men and 11 women) and 19 essential hypertension (EH) patients (58 +/- 2 years, nine men and 10 women). Plasma membrane PLC activity, assessed by conversion of the tritiated exogenous phosphatidylinositol-4,5-bisphosphate to inositol trisphosphate, was greater in EH patients than in normotensive subjects (1.4 +/- 0.2 versus 0.7 +/- 0.1 pmol/mg protein/min, P <0.05). There was a positive correlation between PLC activity and mean blood pressure measured at admission and 7 days after admission (r = 0.47 and 0.37 respectively, both P <0.05). The value of the Michaelis constant was lower in EH patients than in normotensive subjects (32.1 +/- 5.6 versus 58.3 +/- 10.0 micromol/l, P <0.05), despite the fact that maximal velocity of the reaction was no different. Western blot analysis against PLC beta2 and beta3, gamma, delta1, and G protein gamma2 and gamma5 revealed that most PLC and G protein isoforms detected were delta1 of PLC and gamma2 of G protein, and no difference was detected in their amount between two groups. CONCLUSIONS: We conclude that enhanced PLC delta1 activity may be involved in the pathogenesis of human hypertension.
Authors:
Takuo Kosugi; Tomohiro Osanai; Takaatsu Kamada; Takao Nakano; Ken Okumura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of hypertension     Volume:  21     ISSN:  0263-6352     ISO Abbreviation:  J. Hypertens.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-17     Completed Date:  2003-12-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8306882     Medline TA:  J Hypertens     Country:  England    
Other Details:
Languages:  eng     Pagination:  583-90     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
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MeSH Terms
Descriptor/Qualifier:
Case-Control Studies
Cells, Cultured
Female
Fibroblasts / enzymology
GTP-Binding Proteins / metabolism
Humans
Hypertension / enzymology*
Isoenzymes / metabolism
Kinetics
Male
Middle Aged
Phospholipase C delta
Second Messenger Systems
Skin / enzymology
Type C Phospholipases / metabolism*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.11/PLCD1 protein, human; EC 3.1.4.11/Phospholipase C delta; EC 3.6.1.-/GTP-Binding Proteins
Comments/Corrections
Comment In:
J Hypertens. 2003 Mar;21(3):497-9   [PMID:  12640240 ]

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