| Phospholipase C Mediates Cadmium-Dependent Apoptosis in HEK 293 Cells. | |
| | |
MedLine Citation:
|
PMID: 22151885 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Cadmium is a heavy metal that is known to cause toxicity to cells and at low concentrations can initiate apoptosis. The present study was undertaken with the aim of defining the role of phospholipase C (PLC) in mediating cadmium-induced apoptosis in human embryonic kidney HEK 293 cells. We have shown that intracellular Ca(2+) levels increased significantly in HEK 293 cells after 24 hr exposure to Cd. The activity of the calcium-dependent protease calpain rose by 4-fold. The phospholipase C specific inhibitor, U73122, prevented the Cd-dependent increase in Ca(2+) levels and also abolished Cd-dependent calpain and caspase 3 activation as well as Cd-dependent mitochondrial Bax accumulation. Inhibition of PLC also leads to an increased cell viability following exposure to Cd. Taken together, the results show that the PLC pathway is involved in mediating Cd-induced apoptosis in HEK 293 cells. |
| | |
Authors:
|
Akeem O Lawal; Elizabeth M Ellis |
Related Documents
:
|
17507665 - Glutathione redox regulates airway hyperresponsiveness and airway inflammation in mice. 15341185 - Differential role of extra- and intracellular superoxide anions for nitric oxide-mediat... 8304825 - Antioxidants modulate induction of programmed endothelial cell death (apoptosis) by end... 11246125 - Cadmium adaptation in the lung - a double-edged sword? 19514995 - Anti-inflammatory activity of motherwort (leonurus sibiricus l.). 7674335 - Gradual loss of t-helper 1 populations in spleen of mice during progressive tumor growth. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-9 |
Journal Detail:
|
Title: Basic & clinical pharmacology & toxicology Volume: - ISSN: 1742-7843 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101208422 Medline TA: Basic Clin Pharmacol Toxicol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society. |
Affiliation:
|
Strathclyde Institute of Pharmacy and Biomedical Sciences University of Strathclyde, Glasgow, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The so-called "Spanish model" - Tobacco industry strategies and its impact in Europe and Latin Ameri...
Next Document: Effects of Human Immunodeficiency Virus and Metabolic Complications on Myocardial Nutrient Metabolis...