Document Detail


Phospholipase A2-interacting weak neurotoxins from venom of monocled cobra Naja kaouthia display cell-specific cytotoxicity.
MedLine Citation:
PMID:  18456298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular weights of two phospholipase A(2) (PLA(2))-interacting polypeptides (kaouthiotoxins (KTXs)-KTX-A and KTX-B) purified from the venom of monocled cobra Naja kaouthia, were estimated by mass spectrometry as 7722 and 7627Da, respectively. Binary sequence alignment showed that both KTXs share substantial sequence homology with weak neurotoxins from cobra venom and they were devoid of any enzymatic activity. Their pI was determined at pH 8.1 showing basic nature of these proteins. KTXs displayed cell-specific cytotoxicity on mammalian and insect cells.
Authors:
Ashis K Mukherjee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-15
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  51     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  1538-43     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India. akm@tezu.ernet.in
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cercopithecus aethiops
Chemical Fractionation
Cobra*
Cobra Venoms / chemistry*
Cytotoxins / pharmacology*
Hydrogen-Ion Concentration
Insects / drug effects
Neurotoxins / pharmacology*
Phospholipases A2 / pharmacology*
Sequence Alignment
Vero Cells
Chemical
Reg. No./Substance:
0/Cobra Venoms; 0/Cytotoxins; 0/Neurotoxins; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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