| Phospholipase A2 inactivation of microsomal 17 beta-hydroxysteroid oxidoreductase: rates of phospholipid hydrolysis and enzyme inactivation, effects of hydrolysis products and properties of the phospholipase A2-treated enzyme. | |
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MedLine Citation:
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PMID: 6932106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exposure of guinea pig liver microsomes to phospholipase A2 resulted in the nearly complete loss of 17 beta-hydroxysteroid oxidoreductase (17 beta-HSD) activity, the time course of which correlated with phospholipid hydrolysis and lysolecithin formation. Lysolecithin and unsaturated fatty acids added to microsomes also inactivated 17 beta-HSD indicating that they may contribute to the inactivation by phospholipase A2. If exposure to lysolecithin and fatty acids was minimized by including serum albumin in the reaction mixture, phospholipids were rapidly hydrolyzed; but in this case the extent of 17 beta-HSD inactivation was less and the rate of loss was significantly slower. The data suggest that phospholipid hydrolysis per se results in a destabilization of 17 beta-HSD resulting in the subsequent activity loss. The inactivation of 17 beta-HSD by lysolecithin and fatty acids has not been reported previously and is suggestive of a possible control mechanism in vivo. |
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Authors:
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C H Blomquist; C E Kotts; E Y Hakanson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Steroids Volume: 36 ISSN: 0039-128X ISO Abbreviation: Steroids Publication Date: 1980 Jul |
Date Detail:
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Created Date: 1980-11-20 Completed Date: 1980-11-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404536 Medline TA: Steroids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 97-113 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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17-Hydroxysteroid Dehydrogenases
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antagonists & inhibitors* Animals Fatty Acids / pharmacology Guinea Pigs Hydrolysis Lysophosphatidylcholines / biosynthesis, pharmacology Microsomes, Liver / enzymology* Oleic Acids / pharmacology Phospholipases / metabolism* Phospholipases A / metabolism* Phospholipases A2 Phospholipids / metabolism Serum Albumin, Bovine / pharmacology Testosterone / metabolism Time Factors Trypsin |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Oleic Acids; 0/Phospholipids; 0/Serum Albumin, Bovine; 58-22-0/Testosterone; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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