Document Detail


Phospholipase A2 activity is increased in guinea pig uterine cervix in late pregnancy and at parturition.
MedLine Citation:
PMID:  7491947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Release of arachidonic acid from phospholipids by phospholipases A2 (PLA2) is the rate-limiting step in prostaglandin (PG) synthesis. PGE2 and PGF2 alpha are essential intermediates in interstitial collagenase-mediated degradation of type I collagen, a key step in cervical dilatation at parturition. We demonstrate that PLA2 is present in cytosolic fractions of guinea pig cervix and PLA2 activity is increased during cervical dilatation at parturition. In the cervix of nonpregnant animals, PLA2 activity against phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was 13 +/- 6 and 49 +/- 27 pmol.min-1.mg protein-1, respectively. Levels were similar at day 25 of pregnancy. At day 50, PLA2-PC increased to 190 +/- 54 pmol.min-1.mg-1, and PLA2-PE rose to 592 +/- 127 pmol.min-1.mg-1. At parturition (68 +/- 2 days) there were further increases of 49-67%. PLA2 activity declined toward basal levels 2 days postpartum. Almost 50% of the enhanced cytosolic PLA2 (cPLA2) activity at day 50 or at parturition was of high molecular mass and was identified as the "85-kDa cPLA2." Increases in cPLA2 activity at these time points were not, however, associated with increases in cPLA2 protein or phosphorylation of cPLA2, compared with nonpregnant animals. This study suggests that multiple PLA2 enzymes, including cPLA2, are involved in cervical dilatation at parturition.
Authors:
M R Rajabi; A V Cybulsky
Related Documents :
426017 - Whole-blood fibrinolytic activity in normal and hypertensive pregnancies and its relati...
6586207 - Endometrial prostaglandins and phospholipase activity related to implantation in rabbit...
16522637 - Null mutations in drosophila n-acetylglucosaminyltransferase i produce defects in locom...
21555777 - Ear, nose, and throat manifestations during pregnancy.
1396347 - Gastrin releasing peptide immunoreactivity is present in ovine amniotic fluid and fetal...
21228557 - Delayed childbearing: effects on fertility and the outcome of pregnancy.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-04     Completed Date:  1996-01-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E940-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey, Newark 07103, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acids / pharmacology
Cervix Uteri / enzymology*
Chromatography, Gel
Cytosol / enzymology
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors / pharmacology
Female
Guinea Pigs
Immunoblotting
Labor, Obstetric / metabolism*
Phospholipases A / antagonists & inhibitors,  metabolism*
Phospholipases A2
Pregnancy
Pregnancy, Animal / metabolism*
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Enzyme Inhibitors; 149301-79-1/arachidonyltrifluoromethane; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Suppression of circulating calcitriol and duodenal active Ca transport by ketoconazole in pregnant r...
Next Document:  Use of R-beta-[1-11C]hydroxybutyrate in PET studies of regional cerebral uptake of ketone bodies in ...