Document Detail


Phospholipase A2 activity in salivary glands and saliva of the lone star tick (Acari: Ixodidae) during tick feeding.
MedLine Citation:
PMID:  9701935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phospholipase A2 (PLA2) activity levels in tick [Amblyomma americanum (L.)] salivary glands and saliva were examined during tick feeding by using 14C-phosphatidylcholine as the substrate. Saliva produced by stimulating female ticks to salivate with dopamine contains PLA2 (ts-PLA2) activity. The ts-PLA2 activity level in saliva did not change significantly during tick feeding except for a decrease in the last rapid feeding phase (> 200 mg) and in replete ticks. Phospholipase A2 activity was higher in salivary glands of fed than unfed ticks, both in males and females; activity increased during tick feeding correlating with salivary secretory rates during tick feeding suggesting that much of the PLA2 activity measured in whole salivary glands is synthesized for subsequent secretion. During the time course of in vitro salivation, the first 10 microliters of saliva contained higher ts-PLA2 activity than saliva secreted thereafter. Phospolipase A2 was identified in the saliva of artificially fed ticks indicating that ts-PLA2 is a physiological component of tick saliva.
Authors:
K Zhu; A S Bowman; J W Dillwith; J R Sauer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medical entomology     Volume:  35     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-09-16     Completed Date:  1998-09-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  500-4     Citation Subset:  IM    
Affiliation:
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74078, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Feeding Behavior
Female
Phospholipases A / metabolism*
Phospholipases A2
Saliva / enzymology*
Salivary Glands / enzymology
Ticks
Grant Support
ID/Acronym/Agency:
AI-31460/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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