| Phospholipase A2 (PLA2) activity in gerbil brain: characterization of cytosolic and membrane-associated forms and effects of ischemia and reperfusion on enzymatic activity. | |
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MedLine Citation:
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PMID: 8358004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipases A2 comprise a family of enzymes that hydrolyze the acyl bond at the sn-2 position of phospholipids to generate free fatty acids and lysophospholipids. In the central nervous system products of PLA2 regulate neurotransmission. In addition, the lysophospholipids, free fatty acids, eicosanoids, platelet activating factor and reactive oxygen species, generated by enhanced PLA2 activity and arachidonic acid metabolism, may be responsible for many destructive cellular processes in neuronal tissue. There are interactions between glutamate and PLA2 and its products which suggest that PLA2 activity plays an important role in excitotoxic neuronal cell injury associated with ischemia. Our laboratory has demonstrated that multiple forms of Ca(2+)-dependent PLA2 are present in the gerbil brain. These forms differ from previously described forms and from each other. After ischemia and reperfusion, cytosolic, mitochondrial/synaptosomal and microsomal PLA2 enzymatic activities are enhanced. These stable modifications of enzymatic activity cannot be explained by a direct effect of Ca2+ alone and our data suggest that regulatory influences other than Ca2+ may play an important role in PLA2 activation and mediation of cellular injury after an ischemic insult. |
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Authors:
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J V Bonventre; W J Koroshetz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: Journal of lipid mediators Volume: 6 ISSN: 0921-8319 ISO Abbreviation: J Lipid Mediat Publication Date: 1993 Mar-Apr |
Date Detail:
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Created Date: 1993-09-24 Completed Date: 1993-09-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8913460 Medline TA: J Lipid Mediat Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 457-71 Citation Subset: IM |
Affiliation:
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Medical Service, Massachusetts General Hospital, Boston. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / enzymology* Brain Ischemia / enzymology Cytosol / enzymology Gerbillinae Membranes / enzymology Phospholipases A / metabolism* Phospholipases A2 Reperfusion Injury / enzymology |
| Grant Support | |
ID/Acronym/Agency:
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DK 39773/DK/NIDDK NIH HHS; NS 10828/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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