Document Detail


Phosphoinositides, inositol phosphates, and phospholipase C in embryonic stem cells.
MedLine Citation:
PMID:  16845989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The stimulation of inositol phospholipid metabolism via phospholipase C (PLC) is an important signal transduction pathway in a wide variety of cell types. Activation of the pathway is associated with many aspects of cellular activity, including cell growth and differentiation. Activation of hormone-sensitive PLC results in the rapid breakdown of polyphosphoinositides to generate two second messengers: inositol trisphosphate and diacylglycerol. The water-soluble inositol trisphosphate is involved in the release of intracellular calcium from internal stores, whereas the lipophilic diacylglycerol is involved in protein kinase C activation. Inositol supplementation is essential for the in vitro growth of rabbit blastocysts, and studies have shown that the components of the signaling system are present in mouse and cattle embryos and in mouse embryonic stem (ES) cells. In ES cells, the signaling system appears to be constitutively active and essential for normal ES cell proliferation. Here, we describe in detail the materials required and some of techniques involved in studying the phosphoinositide signaling system in mouse ES cells. Furthermore, we describe methods of analyzing the effects of modulating the PtdIns signaling system on ES cell proliferation and the induction of apoptosis.
Authors:
Leo R Quinlan
Related Documents :
16483139 - Cortactin mediated morphogenic cell movements during zebrafish (danio rerio) gastrulation.
5002429 - Determination of blastoderm cells in drosophila melanogaster.
7576119 - Embryo-associated immunosuppressor factor is produced at the maternal-fetal interface i...
11891919 - Nucleolar protein allocation and ultrastructure in bovine embryos produced by nuclear t...
7444199 - Teratocarcinogenesis and spontaneous parthenogenesis in mice.
22754279 - Cigarette smoke extract stimulates rat pulmonary artery smooth muscle cell proliferatio...
3788959 - Kinetic analysis in single, intact cells by microspectrophotometry: evidence for two po...
113649 - Lysosomal enzymes and microbicidal capacity of activated macrophage.
10427129 - Mouse leukemia l1210 cells selected for resistance to the ribonucleotide reductase inhi...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  329     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-18     Completed Date:  2006-08-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-49     Citation Subset:  IM    
Affiliation:
Physiology Department, National University of Ireland, Galway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Cell Culture Techniques / methods
Cell Proliferation
Cell Survival
Cells, Cultured
Embryo, Mammalian / cytology*
Inositol Phosphates / analysis,  metabolism*
Mice
Phosphatidylinositols / analysis,  metabolism*
Signal Transduction
Stem Cells / cytology,  metabolism*
Tritium
Type C Phospholipases / metabolism*
Chemical
Reg. No./Substance:
0/Inositol Phosphates; 0/Phosphatidylinositols; 10028-17-8/Tritium; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Matrix assembly, cell polarization, and cell survival: analysis of peri-implantation development wit...
Next Document:  Cripto signaling in differentiating embryonic stem cells.