Document Detail

Phosphoinositides, inositol phosphates, and phospholipase C in embryonic stem cells.
MedLine Citation:
PMID:  16845989     Owner:  NLM     Status:  MEDLINE    
The stimulation of inositol phospholipid metabolism via phospholipase C (PLC) is an important signal transduction pathway in a wide variety of cell types. Activation of the pathway is associated with many aspects of cellular activity, including cell growth and differentiation. Activation of hormone-sensitive PLC results in the rapid breakdown of polyphosphoinositides to generate two second messengers: inositol trisphosphate and diacylglycerol. The water-soluble inositol trisphosphate is involved in the release of intracellular calcium from internal stores, whereas the lipophilic diacylglycerol is involved in protein kinase C activation. Inositol supplementation is essential for the in vitro growth of rabbit blastocysts, and studies have shown that the components of the signaling system are present in mouse and cattle embryos and in mouse embryonic stem (ES) cells. In ES cells, the signaling system appears to be constitutively active and essential for normal ES cell proliferation. Here, we describe in detail the materials required and some of techniques involved in studying the phosphoinositide signaling system in mouse ES cells. Furthermore, we describe methods of analyzing the effects of modulating the PtdIns signaling system on ES cell proliferation and the induction of apoptosis.
Leo R Quinlan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  329     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-18     Completed Date:  2006-08-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  127-49     Citation Subset:  IM    
Physiology Department, National University of Ireland, Galway.
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MeSH Terms
Cell Culture Techniques / methods
Cell Proliferation
Cell Survival
Cells, Cultured
Embryo, Mammalian / cytology*
Inositol Phosphates / analysis,  metabolism*
Phosphatidylinositols / analysis,  metabolism*
Signal Transduction
Stem Cells / cytology,  metabolism*
Type C Phospholipases / metabolism*
Reg. No./Substance:
0/Inositol Phosphates; 0/Phosphatidylinositols; 10028-17-8/Tritium; EC 3.1.4.-/Type C Phospholipases

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