Document Detail

Phosphoinositide-specific phospholipase Cbeta1 expression is not linked to nerve growth factor-induced differentiation, cell survival or cell cycle control in PC12 rat pheocromocytoma cells.
MedLine Citation:
PMID:  11746516     Owner:  NLM     Status:  MEDLINE    
Recent reports have highlighted that phosphoinositide-specific phospholipase Cbeta1 expression is linked to neuronal differentiation in different experimental models. We sought to determine whether or not this is also true for nerve growth factor (NGF)-induced neuronal differentiation of rat PC12 cells. However, we did not find differences in the expression of both the forms of phosphoinositide-specific phospholipase Cbeta1 (a and b) during sympathetic differentiation of these cells. Also, PC12 cell clones stably overexpressing phosphoinositide-specific phospholipase Cbeta1 were not more susceptible to the differentiating effect of NGF. Furthermore, since it is well established that phosphoinositide-specific phospholipase Cbeta1 affects cell proliferation, we investigated whether or not PC12 cell clones stably overexpressing phosphoinositide-specific phospholipase Cbeta1 showed differences in survival to serum deprivation and cell cycle, when compared to wild type cells. Nevertheless, we did not find any differences in these parameters between wild type cells and the overexpressing clones. Interestingly, in PC12 cells the overexpressed phosphoinositide-specific phospholipase Cbeta1 did not localize to the nucleus, but by immunofluorescence analysis, was detected in the cytoplasm. Therefore, our findings may represent another important clue to the fact that only when it is located within the nucleus phosphoinositide-specific phospholipase Cbeta1 is able to influence cell proliferation.
R Bortul; M Aluigi; P L Tazzari; G Tabellini; G Baldini; R Bareggi; P Narducci; A M Martelli
Related Documents :
21684256 - Pancreatic exocrine enzyme-producing cell differentiation via embryoid bodies from huma...
2113086 - Neuronal properties and trophic activities of immortalized hippocampal cells from embry...
15843036 - Caspase-1 activity is required for neuronal differentiation of pc12 cells: cross-talk b...
16619236 - Hydralazine rescues pc12 cells from acrolein-mediated death.
342596 - An immunocytochemical study of a rat pituitary multipotential clone.
19383896 - The wd40 repeat protein nedd1 functions in microtubule organization during cell divisio...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  84     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-03-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  56-67     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Dipartimento di Morfologia Umana Normale, Università di Trieste, via Manzoni 16, 34138 Trieste, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / physiology
Cell Cycle / drug effects,  physiology
Cell Differentiation / drug effects*,  physiology
Cell Survival / drug effects,  physiology
Culture Media, Serum-Free / metabolism
Cytoplasm / metabolism
Isoenzymes / metabolism*
Nerve Growth Factor / pharmacology*
PC12 Cells / drug effects*,  enzymology,  pathology
Phosphatidylinositols / metabolism*
Phospholipase C beta
Type C Phospholipases / metabolism*
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Isoenzymes; 0/Phosphatidylinositols; 9061-61-4/Nerve Growth Factor; EC 3.1.4.-/Type C Phospholipases; EC C beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Relation between the flexibility of the WPD loop and the activity of the catalytic domain of protein...
Next Document:  Comparative efficiencies of C-terminal signals of native glycophosphatidylinositol (GPI)-anchored pr...