| Phosphoinositide breakdown in isolated myelin is stimulated by GTP analogues and calcium. | |
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MedLine Citation:
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PMID: 1965838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Purified myelin from rat brainstem, prelabeled in vivo by intracerebral injection of [3H]myoinositol, showed enhanced breakdown of phosphoinositides on treatment with 5'-guanylylimidodiphosphate [Gpp-(NH)p] and Ca2+. Concentration variation of the former in the presence of Ca2+ showed a dose-dependent release of inositol 1,4-bisphosphate (IP2) and inositol 1,4,5-trisphosphate (IP3), while inositol 1-phosphate (IP) release was erratic. Concentration-dependent release of IP2 and IP3 was also observed with Ca2+ as the variable in the presence of Gpp(NH)p. Carbachol, when present, did not enhance the stimulatory effect of Gpp(NH)p alone. Addition of diphosphoglycerate during incubation enhanced IP3 at the expense of IP2, suggesting the presence of IP3 phosphatase in myelin. |
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Authors:
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F Golly; J N Larocca; R W Ledeen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroscience research Volume: 27 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 1990 Nov |
Date Detail:
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Created Date: 1991-07-19 Completed Date: 1991-07-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 342-8 Citation Subset: IM |
Affiliation:
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Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / pharmacology* Carbachol / pharmacology Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology Guanosine Triphosphate / physiology* Guanylyl Imidodiphosphate / pharmacology Inositol Phosphates / metabolism Myelin Proteins / metabolism* Phosphatidylinositols / metabolism* Rats Rats, Inbred Strains Receptors, Muscarinic / drug effects Second Messenger Systems |
| Grant Support | |
ID/Acronym/Agency:
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NS 16181/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Myelin Proteins; 0/Phosphatidylinositols; 0/Receptors, Muscarinic; 34273-04-6/Guanylyl Imidodiphosphate; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 51-83-2/Carbachol; 7440-70-2/Calcium; 86-01-1/Guanosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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