Document Detail

Phosphoinositide 3-phosphatase segregates from phosphatidylinositol 3-kinase in EGF-stimulated A431 cells and fails to in vitro hydrolyse phosphatidylinositol(3,4,5)trisphosphate.
MedLine Citation:
PMID:  8137909     Owner:  NLM     Status:  MEDLINE    
Beside 4- and 5-phosphatases playing a role in the interconversion between the D-3 phosphorylated polyphosphoinositides, the only enzyme described so far to be responsible for a phosphomonesterasic activity on the D-3 position of inositol lipids is a specific 3-phosphatase that hydrolyzes PtdIns(3)P in NIH 3T3 cells. We report here the presence of a potent 3-phosphatase activity in different cell types. This activity is detected both in cytosol and membranes of A431 cells and is inhibited by VO4(-3) and Zn2+. Interestingly, the cytosolic activity from A431 cells selectively hydrolyzes in vitro PtdIns(3)P and PtdIns(3,4)P2, whereas PtdIns(3,4,5)P3 remains a very poor substrate under the same conditions. Finally, assays of phosphatidylinositol 3-kinase and 3-phosphatase activities in the pool of phosphotyrosine-containing proteins isolated from EGF-stimulated A431 cells suggest a compartmentation of these two antagonistic activities during cell activation.
B Payrastre; D Gironcel; M Plantavid; G Mauco; M Breton; H Chap
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  341     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-04-28     Completed Date:  1994-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-8     Citation Subset:  IM    
INSERM Unité 326, Hôpital Purpan, Toulouse, France.
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MeSH Terms
1-Phosphatidylinositol 3-Kinase
3T3 Cells
Cell Line
Enzyme Activation
Epidermal Growth Factor / physiology*
Phosphatidylinositol Phosphates / metabolism*
Phosphoric Monoester Hydrolases / metabolism*
Phosphotransferases (Alcohol Group Acceptor) / metabolism*
Substrate Specificity
Reg. No./Substance:
0/Phosphatidylinositol Phosphates; 0/phosphatidylinositol 3,4,5-triphosphate; 62229-50-9/Epidermal Growth Factor; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 3-Kinase; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC

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