| Phosphoinositide 3-kinase inhibitor LY294002 but not serum withdrawal suppresses proliferation of murine embryonic stem cells. | |
| | |
MedLine Citation:
|
PMID: 17321171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Mouse embryonic stem (mES) cells have short duration of their cell cycle and are capable of proliferating in the absence of growth factors. To find out which signaling pathways contribute to the regulation of the mES cell cycle, we used pharmacological inhibitors of MAP and PI3 kinase cascades. The MAP kinase inhibitors as well as serum withdrawal did not affect mES cell cycle distribution, whereas the inhibitor of PI3K activity, LY294002, induced accumulation of cells in G(1) phase followed by apoptotic cell death. Serum withdrawal also causes apoptosis, but it does not change the content and activity of cell cycle regulators. In contrast, in mES cells treated with LY294002, the activities of Cdk2 and E2F were significantly decreased. Interestingly, LY294002had a much stronger effect on cell cycle distribution in low serum conditions, implying that serum can promote G(1)-->S transition of mES cells by a LY294002-resistant mechanism. Thus, proliferation of mES cells is maintained by at least two separate mechanisms: a LY294002-sensitive pathway, which is active even in the absence of serum, and LY294002-resistant, but serum-dependent, pathway. |
| | |
Authors:
|
Maria S Lianguzova; Ilia A Chuykin; Alfred Nordheim; Valery A Pospelov |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-21 |
Journal Detail:
|
Title: Cell biology international Volume: 31 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 2007 Apr |
Date Detail:
|
Created Date: 2007-04-02 Completed Date: 2007-06-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 330-7 Citation Subset: IM |
Affiliation:
|
Institute of Cytology, RAS, St. Petersburg 194064, Russian Federation. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Phosphatidylinositol 3-Kinase
/
antagonists & inhibitors* Animals Cell Cycle / drug effects Cell Proliferation / drug effects* Cells, Cultured / drug effects Chromones / pharmacology* Culture Media, Serum-Free* E2F Transcription Factors / metabolism Embryonic Stem Cells / drug effects* Enzyme Inhibitors / pharmacology* Mice Morpholines / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Chromones; 0/Culture Media, Serum-Free; 0/E2F Transcription Factors; 0/Enzyme Inhibitors; 0/Morpholines; 154447-36-6/2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neuroprotective effects of tetramethylpyrazine on hydrogen peroxide-induced apoptosis in PC12 cells.
Next Document: Progenitor cells for cardiac repair.