| Phosphodiesterase inhibitor 3-isobutyl-methyl-xanthine affects rabbit ovaries and oviduct. | |
| | |
MedLine Citation:
|
PMID: 20599929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of our study was to examine the influence of 3-isobutyl-1-methyl-xanthine (IBMX), an inhibitor of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) phosphodiesterases on rabbit reproductive system. The ovarian cycle and ovulation of control rabbits were induced by gonadotropin releasing hormone (GnRH) or equine chorionic gonadotropin (eCG) followed by the human chorionic gonadotropin (hCG) administration. Experimental animals received GnRH together with IBMX (at 50 or 500 microg/animal) or eCG and hCG together with IBMX (at 5 or 257 microg/animal). After mating and insemination, blood samples were collected and analyzed for concentrations of progesterone and estradiol by RIA; some animals from the control and IBMX-treated groups were killed. The presence of follicles at different stages of development was evaluated by a light microscopy. Isolated ovarian fragments were cultured for 2 days. The secretion of progesterone and estradiol was assessed by RIA. The expression of markers of proliferation (PCNA and cyclin B1) and apoptosis (bax) in ovarian fragments was evaluated by Western-blotting. Epithelial cells were isolated from oviducts and cultured for 2 days. The expression of markers of proliferation (PCNA, ERK1,2-related MAP kinase) and apoptosis (bax, bcl-2) in the oviductal cells was evaluated by immunocytochemistry. It was observed, that the ovaries of rabbits treated with IBMX contained more secondary follicles, than control rabbits. Administration of IBMX reduced blood level of progesterone, but did not affect blood estradiol. Fragments of ovaries isolated from rabbits treated with IBMX released more estradiol, but not progesterone, than ovarian cells isolated from the control animals. IBMX injections substantially decreased the expression of the upper (23 kD), but not the bottom (24 kD) fraction of bax. IBMX administration did not affect PCNA, but it caused a decrease in the upper fraction (54 kD) and an increase in the bottom fraction (55 kD) of cyclin B1. Oviductal cells isolated from the IBMX-treated animals, contained less marker of apoptosis - bax (but not bcl-2) and proliferation - ERK1,2-related MAP kinase (but not PCNA) than control animals. These observations demonstrate the involvement of cyclic nucleotide-dependent intracellular mechanisms in control of rabbit reproductive functions: ovarian folliculogenesis, apoptosis, proliferation and steroid hormone release, as well as proliferation and apoptosis of the oviductal cells. |
| | |
Authors:
|
Alexander V Sirotkin; Peter Chrenek; Stella Chadio; Eftichia Xylouri; Helen Fotopouloy; Alexander V Makarevich |
Related Documents
:
|
12185589 - Prb2/p130 promotes radiation-induced cell death in the glioblastoma cell line hjc12 by ... 14742319 - Id-1-induced raf/mek pathway activation is essential for its protective role against ta... 10333359 - Inverse relationship between apoptosis and bcl-2 expression in syncytiotrophoblast and ... 12387829 - Bcl-2 can promote p53-dependent senescence versus apoptosis without affecting the g1/s ... 12538159 - Stronger correlation of bcl-3 than bcl-2, bcl-xl, costimulation, or antioxidants with a... 9326359 - Dexamethasone suppresses apoptosis in a human gastric cancer cell line through modulati... 110689 - Effect of type-specific active immunization on the development and progression of exper... 16894359 - The role of toll-like receptors in non-infectious lung injury. 21421889 - Recurrent generalized tetanus: a case report. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-17 |
Journal Detail:
|
Title: European journal of pharmacology Volume: 643 ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-07-26 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 145-51 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Institute of Animal Genetics and Reproduction, Animal Production Research Centre Nitra, 95141 Luzianky near Nitra, Slovak Republic. sirotkin@cvzv.sk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Methyl-3-isobutylxanthine
/
pharmacology* 3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors 3',5'-Cyclic-GMP Phosphodiesterases / antagonists & inhibitors Animals Apoptosis / drug effects Cell Proliferation / drug effects Estrous Cycle / drug effects Female Ovarian Follicle / drug effects, enzymology, pathology Ovary / drug effects*, enzymology, pathology Oviducts / drug effects*, enzymology, pathology Ovulation / drug effects Phosphodiesterase Inhibitors / pharmacology* Rabbits |
| Chemical | |
Reg. No./Substance:
|
0/Phosphodiesterase Inhibitors; 28822-58-4/1-Methyl-3-isobutylxanthine; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases; EC 3.1.4.35/3',5'-Cyclic-GMP Phosphodiesterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Caffeic acid phenethyl ester-mediated Nrf2 activation and IkappaB kinase inhibition are involved in ...
Next Document: Midcervical vagotomy precludes respiratory response to novel anti-inflammatory and anti-tumour drug ...