| Phosphocreatine as an energy source for actin cytoskeletal rearrangements during myoblast fusion. | |
| | |
MedLine Citation:
|
PMID: 18420707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Myoblast fusion is essential for muscle development, postnatal growth and muscle repair after injury. Recent studies have demonstrated roles for actin polymerization during myoblast fusion. Dynamic cytoskeletal assemblies directing cell-cell contact, membrane coalescence and ultimately fusion require substantial cellular energy demands. Various energy generating systems exist in cells but the partitioning of energy sources during myoblast fusion is unknown. Here, we demonstrate a novel role for phosphocreatine (PCr) as a spatiotemporal energy buffer during primary mouse myoblast fusion with nascent myotubes. Creatine treatment enhanced cell fusion in a creatine kinase (CK)-dependent manner suggesting that ATP-consuming reactions are replenished through the PCr/CK system. Furthermore, selective inhibition of actin polymerization prevented myonuclear addition following creatine treatment. As myotube formation is dependent on cytoskeletal reorganization, our findings suggest that PCr hydrolysis is coupled to actin dynamics during myoblast fusion. We conclude that myoblast fusion is a high-energy process, and can be enhanced by PCr buffering of energy demands during actin cytoskeletal rearrangements in myoblast fusion. These findings implicate roles for PCr as a high-energy phosphate buffer in the fusion of multiple cell types including sperm/oocyte, trophoblasts and macrophages. Furthermore, our results suggest the observed beneficial effects of oral creatine supplementation in humans may result in part from enhanced myoblast fusion. |
| | |
Authors:
|
Roddy S O'Connor; Craig M Steeds; Robert W Wiseman; Grace K Pavlath |
Related Documents
:
|
565787 - Transfer of human chromosomes via human minisegregant cells into mouse cells and the qu... 15010527 - Characterization of severe acute respiratory syndrome-associated coronavirus (sars-cov)... 9611827 - Biotransformation of digitoxigenin by cultured strophanthus hybrid cells. 1849407 - Regulated expression of cytochrome p-450scc (cholesterol-side-chain cleavage enzyme) in... 17211937 - "the adipocyte: a multifunctional cell". 7777587 - Establishment and characterization of human fetal liver epithelial cell line transfecte... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-04-17 |
Journal Detail:
|
Title: The Journal of physiology Volume: 586 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-06-16 Completed Date: 2008-08-21 Revised Date: 2012-04-17 |
Medline Journal Info:
|
Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
|
Languages: eng Pagination: 2841-53 Citation Subset: IM |
Affiliation:
|
Emory University, Department of Pharmacology, 1510 Clifton Rd, Room 5027, Atlanta, GA 30322, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actins
/
physiology*,
ultrastructure Animals Cell Fusion Cells, Cultured Creatine Kinase / metabolism* Cytoskeleton / physiology*, ultrastructure* Humans Mice Mice, Inbred BALB C Myoblasts / cytology*, physiology* Phosphocreatine / metabolism* Signal Transduction / physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
AR047314/AR/NIAMS NIH HHS; AR051372/AR/NIAMS NIH HHS; AR052730/AR/NIAMS NIH HHS; R01 AR047314-10/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 67-07-2/Phosphocreatine; EC 2.7.3.2/Creatine Kinase |
| Comments/Corrections | |
Comment In:
|
J Physiol. 2008 Jun 15;586(Pt 12):2817-8
[PMID:
18556720
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: How do tonic glutamatergic synapses evade receptor desensitization?
Next Document: Role of sarcolemmal ATP-sensitive K+ channels in the regulation of sinoatrial node automaticity: an ...