Document Detail

Phosphocreatine as an energy source for actin cytoskeletal rearrangements during myoblast fusion.
MedLine Citation:
PMID:  18420707     Owner:  NLM     Status:  MEDLINE    
Myoblast fusion is essential for muscle development, postnatal growth and muscle repair after injury. Recent studies have demonstrated roles for actin polymerization during myoblast fusion. Dynamic cytoskeletal assemblies directing cell-cell contact, membrane coalescence and ultimately fusion require substantial cellular energy demands. Various energy generating systems exist in cells but the partitioning of energy sources during myoblast fusion is unknown. Here, we demonstrate a novel role for phosphocreatine (PCr) as a spatiotemporal energy buffer during primary mouse myoblast fusion with nascent myotubes. Creatine treatment enhanced cell fusion in a creatine kinase (CK)-dependent manner suggesting that ATP-consuming reactions are replenished through the PCr/CK system. Furthermore, selective inhibition of actin polymerization prevented myonuclear addition following creatine treatment. As myotube formation is dependent on cytoskeletal reorganization, our findings suggest that PCr hydrolysis is coupled to actin dynamics during myoblast fusion. We conclude that myoblast fusion is a high-energy process, and can be enhanced by PCr buffering of energy demands during actin cytoskeletal rearrangements in myoblast fusion. These findings implicate roles for PCr as a high-energy phosphate buffer in the fusion of multiple cell types including sperm/oocyte, trophoblasts and macrophages. Furthermore, our results suggest the observed beneficial effects of oral creatine supplementation in humans may result in part from enhanced myoblast fusion.
Roddy S O'Connor; Craig M Steeds; Robert W Wiseman; Grace K Pavlath
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-04-17
Journal Detail:
Title:  The Journal of physiology     Volume:  586     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-16     Completed Date:  2008-08-21     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2841-53     Citation Subset:  IM    
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MeSH Terms
Actins / physiology*,  ultrastructure
Cell Fusion
Cells, Cultured
Creatine Kinase / metabolism*
Cytoskeleton / physiology*,  ultrastructure*
Mice, Inbred BALB C
Myoblasts / cytology*,  physiology*
Phosphocreatine / metabolism*
Signal Transduction / physiology*
Grant Support
Reg. No./Substance:
0/Actins; 020IUV4N33/Phosphocreatine; EC Kinase
Comment In:
J Physiol. 2008 Jun 15;586(Pt 12):2817-8   [PMID:  18556720 ]

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