Document Detail


Phospho-regulation of DDA3 function in mitosis.
MedLine Citation:
PMID:  20117088     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DDA3 is a microtubule-associated protein that controls chromosome congression and segregation by regulating the mitotic spindle. Depletion of DDA3 alters spindle structure, generates unaligned chromosomes at metaphase, and delays the mitotic progression. Through a mass spectrometry analysis, we found that DDA3 is phosphorylated on Ser225 during mitosis. Phosphorylation of this residue is important for the mitotic function of DDA3, as the phospho-mimicking DDA3-S225D variant, but not the nonphosphorable DDA3-S225A mutant, rescues the DDA3-knockdown phenotype. We conclude that the mitotic function of DDA3 is regulated by phosphorylation on the Ser225 residue.
Authors:
Chang-Young Jang; Judith A Coppinger; John R Yates; Guowei Fang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-01
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  393     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-03-18     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-63     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Chromosome Pairing
HeLa Cells
Humans
Mitosis*
Molecular Sequence Data
Phosphoproteins / genetics,  metabolism*
Phosphorylation / genetics
Serine / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
GM062852/GM/NIGMS NIH HHS; HL079442/HL/NHLBI NIH HHS; P41 RR011823/RR/NCRR NIH HHS; P41 RR011823-13/RR/NCRR NIH HHS; R01 HL079442/HL/NHLBI NIH HHS; R01 HL079442-07/HL/NHLBI NIH HHS; RR11823-10/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/PSRC1 protein, human; 0/Phosphoproteins; 452VLY9402/Serine
Comments/Corrections

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