Document Detail


Phosphatidylseryl-transfer RNA in Bacillus licheniformis 749/C.
MedLine Citation:
PMID:  36914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylserine has been found in extracts of Bacillus licheniformis made under alkaline conditions but not under neutral or acidic ones and was derived from the tRNA fraction. In tRNA preparations kept below neutrality during purification, phosphatidylserine was the only phospholipid released when the pH was raised to 9.0. The amount of bound phosphatidylserine could be increased by incubating tRNA from B. licheniformis or Escherichia coli with CTP and phosphatidic acid in the presence of an S-30 extract from either organism. The tRNA carrying phosphatidylserine has been separated from the bulk of the tRNA by DEAE-Sephadex chromatography in the presence of a detergent. On deaminoacylation of this material and rechromatography on DEAE-Sephadex, a number of peaks were found, indicating that this behavior is not confined to a single isoaccepting species.
Authors:
B N Dancer; J O Lampen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  562     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  462-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus / metabolism*
Hydrogen-Ion Concentration
Penicillinase / biosynthesis*
Phosphatidylserines / metabolism*
RNA, Bacterial / metabolism
RNA, Transfer / analysis,  metabolism*
beta-Lactamases / biosynthesis*
Chemical
Reg. No./Substance:
0/Phosphatidylserines; 0/RNA, Bacterial; 9014-25-9/RNA, Transfer; EC 3.5.2.-/Penicillinase; EC 3.5.2.6/beta-Lactamases

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