Document Detail

Phosphatidylseryl-transfer RNA in Bacillus licheniformis 749/C.
MedLine Citation:
PMID:  36914     Owner:  NLM     Status:  MEDLINE    
Phosphatidylserine has been found in extracts of Bacillus licheniformis made under alkaline conditions but not under neutral or acidic ones and was derived from the tRNA fraction. In tRNA preparations kept below neutrality during purification, phosphatidylserine was the only phospholipid released when the pH was raised to 9.0. The amount of bound phosphatidylserine could be increased by incubating tRNA from B. licheniformis or Escherichia coli with CTP and phosphatidic acid in the presence of an S-30 extract from either organism. The tRNA carrying phosphatidylserine has been separated from the bulk of the tRNA by DEAE-Sephadex chromatography in the presence of a detergent. On deaminoacylation of this material and rechromatography on DEAE-Sephadex, a number of peaks were found, indicating that this behavior is not confined to a single isoaccepting species.
B N Dancer; J O Lampen
Related Documents :
11343794 - Synthesis and hydrolysis by cathepsin b of fluorogenic substrates with the general stru...
17538924 - Amino acids: aspects of impurity profiling by means of ce.
17890314 - Amino acid toxicities of escherichia coli that are prevented by leucyl-trna synthetase ...
10549284 - Transfer rna-dependent translocation of misactivated amino acids to prevent errors in p...
22050284 - Electrochemical determination of pka of n-bases in ionic liquid media.
10899874 - Failure to detect muramic acid in normal rat tissues but detection in cerebrospinal flu...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  562     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  462-70     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacillus / metabolism*
Hydrogen-Ion Concentration
Penicillinase / biosynthesis*
Phosphatidylserines / metabolism*
RNA, Bacterial / metabolism
RNA, Transfer / analysis,  metabolism*
beta-Lactamases / biosynthesis*
Reg. No./Substance:
0/Phosphatidylserines; 0/RNA, Bacterial; 9014-25-9/RNA, Transfer; EC 3.5.2.-/Penicillinase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enzymatic reduction and methylation of folate following pH-dependent, carrier-mediated transport in ...
Next Document:  Polynucleotides XLVII. Synthesis and properties of poly(2-methylthio- and 2-ethylthioadenylic acid)....