Document Detail

Phosphatidylserine- and integrin-mediated phagocytosis of apoptotic luteal cells by macrophages of the rat.
MedLine Citation:
PMID:  15840000     Owner:  NLM     Status:  MEDLINE    
Corpora lutea disappear from ovaries in the absence of conception. The present study was undertaken to examine the hypothesis that disappearance of corpora lutea is accomplished through apoptosis-dependent phagocytosis of luteal cells. When bone marrow cells expressing green fluorescence protein were transplanted into X-ray-irradiated mice, macrophages derived from donor mice were detected within corpora lutea, suggesting macrophage infiltration into the tissue. Dispersed rat luteal cells underwent spontaneous apoptosis during culture and were phagocytosed by luteal macrophages. Treatment with doxorubicin increased the extent of apoptosis in luteal cells, and those cells were more efficiently phagocytosed than cells left untreated. The phagocytosis was inhibited by liposomes containing phosphatidylserine or a peptide containing the integrin-targeted sequence, and was stimulated by milk fat globule epidermal growth factor 8. These results collectively indicate that apoptotic luteal cells are phagocytosed by macrophages in a manner mediated by phosphatidylserine and integrin.
Shinya Kato; Akiko Shiratsuchi; Kaz Nagaosa; Yoshinobu Nakanishi
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  47     ISSN:  0012-1592     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-20     Completed Date:  2005-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa 920-1192, Japan.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Blotting, Western
Bone Marrow Transplantation
Corpus Luteum / cytology*,  physiology
Doxorubicin / pharmacology*
Green Fluorescent Proteins
Image Processing, Computer-Assisted
Integrins / metabolism
Liposomes / metabolism
Macrophages / drug effects,  physiology*
Microscopy, Fluorescence
Milk Proteins / metabolism
Phagocytosis / drug effects,  physiology*
Phosphatidylserines / metabolism
Reg. No./Substance:
0/Integrins; 0/Liposomes; 0/Milk Proteins; 0/Phosphatidylserines; 147336-22-9/Green Fluorescent Proteins; 23214-92-8/Doxorubicin

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