Document Detail


Phosphatidylserine functions as the major precursor of phosphatidylethanolamine in cultured BHK-21 cells.
MedLine Citation:
PMID:  6425837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulse-chase experiments with [3H]serine provide evidence that significant amounts of phosphatidylserine turn over to form phosphatidylethanolamine in mammalian cells in tissue culture. Phospholipase C hydrolysis of [3H]phosphatidylethanolamine synthesized from [3H]serine by baby hamster kidney (BHK-21) cells demonstrates that nearly all of the radiolabel remains in the ethanolamine moiety. Uniform labeling experiments with [3H]serine further demonstrate that the distribution of radiolabel in phosphatidylserine and phosphatidylethanolamine is nearly identical to the mass ratio of these lipids. Physiological concentrations of ethanolamine (20 microM) have only a marginal effect upon the ability of cells in culture to incorporate radiolabeled serine into either phosphatidylserine or phosphatidylethanolamine. These data provide compelling evidence that phosphatidylethanolamine synthesis via phosphatidylserine and phosphatidylserine decarboxylase contributes significantly to membrane biogenesis in mammalian cells.
Authors:
D R Voelker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-06-11     Completed Date:  1984-06-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2669-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cricetinae
Ethanolamines / metabolism
Phosphatidylethanolamines / biosynthesis*
Phosphatidylserines / metabolism*
Serine / metabolism
Grant Support
ID/Acronym/Agency:
GM 32453/GM/NIGMS NIH HHS; RR 05842/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Ethanolamines; 0/Phosphatidylethanolamines; 0/Phosphatidylserines; 56-45-1/Serine
Comments/Corrections

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