Document Detail

Phosphatidylserine externalization in sickle red blood cells: associations with cell age, density, and hemoglobin F.
MedLine Citation:
PMID:  12609840     Owner:  NLM     Status:  MEDLINE    
Phosphatidylserine (PS) is normally confined to the cytoplasmic leaflet of the red blood cell (RBC) membrane, but some sickle RBCs expose PS in the outer leaflet (PS+ cells). This study examined the relationships among PS externalization, fetal hemoglobin content, hydration state, and cell age. Sickle RBCs exhibit a wide range of PS externalization. Those with low-level exposure (type 1 PS+) include many young transferrin-receptor-positive (TfR+) cells. This is not specific for sickle cell disease because many nonsickle TfR+ cells are also PS+. RBCs with higher PS exposure (type 2 PS+) appear to be more specific for sickle cell disease. Their formation is most likely sickling dependent because type 2 PS+ dense sickle cells have a lower percentage of fetal hemoglobin (HbF) than PS- cells in the same density fraction (1.7 vs 2.9; n = 8; P <.01). In vivo experiments using biotin-labeled sickle cells showed a sharp decrease in the percentage of circulating, labeled PS+ cells in the first 24 hours after reinfusion. This decrease was confined to type 1 PS+ cells and was thus consistent with the reversal of PS exposure in very young cells. As the labeled cells aged in the circulation, the percentages of type 1 and type 2 PS+ cells increased. These studies indicate that PS externalization in sickle cells may be low level, as observed in many immature cells, or high level, which is associated with dehydration and appears to be more specific for sickle RBCs.
Zahida Yasin; Scott Witting; Mary B Palascak; Clinton H Joiner; Donald L Rucknagel; Robert S Franco
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-02-27
Journal Detail:
Title:  Blood     Volume:  102     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-19     Completed Date:  2003-08-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  365-70     Citation Subset:  AIM; IM    
Vontz Center for Molecular Studies, Hematology/Oncology Division ML#0508, Department of Internal Medicine, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH, USA.
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MeSH Terms
Anemia, Sickle Cell / blood*
Erythrocyte Aging
Erythrocyte Membrane / metabolism,  ultrastructure
Erythrocytes / chemistry*,  ultrastructure
Fetal Hemoglobin / analysis
Phosphatidylserines / analysis,  metabolism*
Receptors, Transferrin / analysis
Reticulocytes / chemistry,  ultrastructure
Water / metabolism
Grant Support
Reg. No./Substance:
0/Phosphatidylserines; 0/Receptors, Transferrin; 7732-18-5/Water; 9034-63-3/Fetal Hemoglobin

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