| Phosphatidylserine exposure in Fas type I cells is mitochondria-dependent. | |
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MedLine Citation:
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PMID: 12804759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have demonstrated that Fas-triggered activation of effector caspases and subsequent nuclear apoptosis either is mitochondria-independent (type I cells) or relies on mitochondrial amplification of the initial stimulus (type II cells). We show herein that Bcl-2 overexpression in a prototypic type I cell line (SKW6.4) promotes mitochondrial generation of ATP and blocks Fas-triggered plasma membrane externalization of phosphatidylserine (PS). Moreover, overexpression of Bcl-2 attenuates macrophage engulfment of Fas-triggered cells. Fas-mediated DNA fragmentation, on the other hand, remains unaffected in SKW6.4-bcl-2 cells. These studies thus demonstrate that PS externalization and clearance of cell corpses are mitochondria-dependent events, and show that these events can be dissociated from other features of the apoptotic program, in Fas type I cells. |
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Authors:
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Wanlaya Uthaisang; Leta K Nutt; Sten Orrenius; Bengt Fadeel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 545 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-06-13 Completed Date: 2003-07-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 110-4 Citation Subset: IM |
Affiliation:
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Institute of Environmental Medicine, Division of Toxicology, Nobels väg 13, Karolinska Institutet, 171 77, Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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biosynthesis Antibodies, Monoclonal / metabolism Antigens, CD95 / metabolism* Apoptosis Caspases / metabolism Cell Line Cell Membrane / metabolism DNA Fragmentation Humans Macrophages / metabolism Membrane Proteins / metabolism Mitochondria / metabolism* Monocytes / cytology, metabolism* Phagocytosis Phosphatidylserines / metabolism* Proto-Oncogene Proteins c-bcl-2 / metabolism Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/Membrane Proteins; 0/Phosphatidylserines; 0/Proto-Oncogene Proteins c-bcl-2; 56-65-5/Adenosine Triphosphate; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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