Document Detail


Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells by normal human microglia, astrocytes, and glioma cells.
MedLine Citation:
PMID:  11302338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptotic cells display signals that trigger phagocytic removal by macrophages or neighboring cells. To better understand the signals triggering phagocytosis of apoptotic glioma cells, and to identify the cells that might be involved in the phagocytic process, U-251 MG glioma cells were made apoptotic by etoposide (25 microg/ml) treatment and were incubated with normal human astrocytes (NHA), glioma cells, or microglia. Extent of phagocytosis was assessed by an in vitro phagocytosis assay. After 3 h of incubation with apoptotic cells, phagocytes tested were washed to remove nonengulfed cells, then fixed, stained, and counted to determine phagocytosis index (PI). NHA, glioma cells, and microglia all phagocytosed apoptotic, but not nonapoptotic, glioma cells. Microglia, however, had a PI approximately 4-fold higher than did either NHA or glioma cells. Binding of phosphatidylserine (PS) on apoptotic glioma cell membranes by annexin-V inhibited phagocytosis by 90% in both microglia and NHA. The activity of an enzyme (scramblase) that moves PS from the inner cell membrane to the outer cell membrane was also increased in apoptotic glioma cells. These results suggest that a variety of cells present in and near gliomas in vivo can remove glioma cells in a PS-dependent scramblase-mediated fashion. Manipulation of scramblase and/or PS exposure in glioma cells may therefore be a means of triggering phagocytic removal of glioma cells.
Authors:
G H Chang; N M Barbaro; R O Pieper
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuro-oncology     Volume:  2     ISSN:  1522-8517     ISO Abbreviation:  Neuro-oncology     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2001-04-13     Completed Date:  2001-05-03     Revised Date:  2008-11-20    
Medline Journal Info:
Nlm Unique ID:  100887420     Medline TA:  Neuro Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  174-83     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, University of California at San Francisco 94115, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Astrocytes / physiology*
Brain / cytology
Carrier Proteins / metabolism
Cell Line
Glioma / enzymology,  pathology,  physiopathology*
Humans
Membrane Proteins / metabolism
Microglia / physiology*
Phagocytosis / drug effects,  physiology*
Phosphatidylserines / pharmacology,  physiology*
Phospholipid Transfer Proteins*
Reference Values
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Proteins; 0/Phosphatidylserines; 0/Phospholipid Transfer Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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