| Phosphatidylserine dynamics in cellular membranes. | |
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MedLine Citation:
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PMID: 22496416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Much has been learned about the role of exofacial phosphatidylserine (PS) in apoptosis and blood clotting using annexin V. However, because annexins are impermeant and unable to bind PS at low calcium concentration, they are unsuitable for intracellular use. Thus little is known about the topology and dynamics of PS in the endomembranes of normal cells. We used two new probes-green fluorescent protein (GFP)-LactC2, a genetically encoded fluorescent PS biosensor, and 1-palmitoyl-2-(dipyrrometheneboron difluoride)undecanoyl-sn-glycero-3-phospho-L-serine (TopFluor-PS), a synthetic fluorescent PS analogue-to examine PS distribution and dynamics inside live cells. The mobility of PS was assessed by a combination of advanced optical methods, including single-particle tracking and fluorescence correlation spectroscopy. Our results reveal the existence of a sizable fraction of PS with limited mobility, with cortical actin contributing to the confinement of PS in the plasma membrane. We were also able to measure the dynamics of PS in endomembrane organelles. By targeting GFP-LactC2 to the secretory pathway, we detected the presence of PS in the luminal leaflet of the endoplasmic reticulum. Our data provide new insights into properties of PS inside cells and suggest mechanisms to account for the subcellular distribution and function of this phospholipid. |
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Authors:
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Jason G Kay; Mirkka Koivusalo; Xiaoxiao Ma; Thorsten Wohland; Sergio Grinstein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-04-11 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 23 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-05-31 Completed Date: 2012-09-24 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2198-212 Citation Subset: IM |
Affiliation:
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Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Cell Membrane / metabolism* Cholesterol / metabolism Cytosol / metabolism Diffusion Endoplasmic Reticulum / metabolism Fluorescence Recovery After Photobleaching Fluorescent Dyes / chemistry, metabolism Green Fluorescent Proteins / metabolism HeLa Cells Humans Intracellular Membranes / metabolism Lipid Bilayers / metabolism Liposomes / metabolism Phosphatidylserines / metabolism* Protein Binding Recombinant Fusion Proteins / metabolism Spectrometry, Fluorescence |
| Grant Support | |
ID/Acronym/Agency:
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MOP7075//Canadian Institutes of Health Research; MOP93634//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Lipid Bilayers; 0/Liposomes; 0/Phosphatidylserines; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 57-88-5/Cholesterol |
| Comments/Corrections | |
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