Document Detail


Phosphatidylinositol turnover is not a general regulator of neuroblastoma cell differentiation: comparison between two differentiating agents, retinoic acid and gamma-interferon.
MedLine Citation:
PMID:  2492954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The turnover of phosphatidylinositol (PI) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including differentiating agents; however decisive evidence for the idea has not been obtained. In the present paper, we investigated the involvement of PI turnover in cell differentiation using a human neuroblastoma cell line, LAN-1, which can be induced to differentiate along the neuronal pathway by both retinoic acid (RA) and gamma-interferon (gamma-IFN). Analysis of labelled phosphatidylinositol metabolites from prelabelled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol within 1 min of induction of LAN-1 cell differentiation by RA, while no changes were observed in gamma-IFN-treated cells. These findings indicate the occurrence of decreased inositol phospholipid turnover in RA-treated LAN-1 cells and suggest that phosphoinositide-derived metabolites may not constitute general regulators of cellular differentiation.
Authors:
M Lanciotti; P Cornaglia-Ferraris; M Ponzoni
Related Documents :
2602914 - Rheumatoid synovial fluid reconstitutes the b-cell defect in cba/n mice.
7964484 - The t helper cell response in lyme arthritis: differential recognition of borrelia burg...
15778354 - Ex vivo characterization of the autoimmune t cell response in the hla-dr1 mouse model o...
11840454 - Inflammation is preceded by tumor necrosis factor-dependent infiltration of mesenchymal...
16585264 - Endogenous irbp can be dispensable for generation of natural cd4+cd25+ regulatory t cel...
6985154 - Induction of 19s igm secretion in a murine pre-b cell line, 70z/3, by cell hybridizatio...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  243     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-03     Completed Date:  1989-04-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  285-8     Citation Subset:  IM    
Affiliation:
Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Genoa, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / drug effects*
Chromatography, High Pressure Liquid / methods
Chromatography, Thin Layer
Humans
Interferon-gamma / pharmacology*
Neuroblastoma
Phosphatidylinositols / metabolism*,  physiology
Tretinoin / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Phosphatidylinositols; 302-79-4/Tretinoin; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Epinephrine and the Ca2+ ionophore A23187 synergistically induce platelet aggregation without protei...
Next Document:  Variability in electrophoretic mobility of Gi-like proteins: effect of SDS.