Document Detail

Phosphatidylinositol turnover is not a general regulator of neuroblastoma cell differentiation: comparison between two differentiating agents, retinoic acid and gamma-interferon.
MedLine Citation:
PMID:  2492954     Owner:  NLM     Status:  MEDLINE    
The turnover of phosphatidylinositol (PI) is believed to constitute a crucial step in the signaling pathways for stimulation of cells by a variety of bioactive substances, including differentiating agents; however decisive evidence for the idea has not been obtained. In the present paper, we investigated the involvement of PI turnover in cell differentiation using a human neuroblastoma cell line, LAN-1, which can be induced to differentiate along the neuronal pathway by both retinoic acid (RA) and gamma-interferon (gamma-IFN). Analysis of labelled phosphatidylinositol metabolites from prelabelled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol within 1 min of induction of LAN-1 cell differentiation by RA, while no changes were observed in gamma-IFN-treated cells. These findings indicate the occurrence of decreased inositol phospholipid turnover in RA-treated LAN-1 cells and suggest that phosphoinositide-derived metabolites may not constitute general regulators of cellular differentiation.
M Lanciotti; P Cornaglia-Ferraris; M Ponzoni
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  243     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-03     Completed Date:  1989-04-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  285-8     Citation Subset:  IM    
Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Genoa, Italy.
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MeSH Terms
Cell Differentiation / drug effects*
Chromatography, High Pressure Liquid / methods
Chromatography, Thin Layer
Interferon-gamma / pharmacology*
Phosphatidylinositols / metabolism*,  physiology
Tretinoin / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Phosphatidylinositols; 302-79-4/Tretinoin; 82115-62-6/Interferon-gamma

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