| Phosphatidylinositol-specific-phospholipase C cleaves urokinase plasminogen activator receptor from the cell surface and leads to inhibition of pemphigus-IgG-induced acantholysis in DJM-1 cells, a squamous cell carcinoma line. | |
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MedLine Citation:
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PMID: 11422178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We showed previously that pemphigus IgG enhanced both the activity of urokinase plasminogen activator (uPA) in cultured cells and the expression of its receptor (uPAR) on uPA-binding keratinocytes. In the present study, to clarify whether uPAR and uPA-activated plasmin are actually involved in the blistering process after pemphigus IgG binding to the cell surface, we examined the effects of the following on uPAR expression and on cell-cell detachment in DJM-1 cells, a squamous cell carcinoma line: (i) phosphatidylinositol-specific phospholipase C (PI-PLC) - which releases uPAR from the membrane surface into the culture medium by cleaving the glycosylphosphatidylinositol anchor thus inhibiting uPAR activity, and (ii) uPA inhibitors (tranexamic acid, aprotinin, p-aminobenzonic acid and dexamethasone). Preincubation with PI-PLC decreased dramatically the pemphigus IgG-induced uPAR expression in a dose-dependent manner, and inhibited pemphigus IgG-induced cell-cell detachment at 10 microg/mL. On the other hand, tranexamic acid (15 mM) inhibited pemphigus IgG-induced cell-cell detachment without reduction of uPAR expression, although aprotinin, p-aminobenzonic acid and dexamethasone failed to alter either of these parameters. Although uPAR expression on the pemphigus IgG-bound cell surface and uPA activation may contribute significantly to the pathogenesis of acantholysis in pemphigus, the mechanisms are complicated and should be defined further. |
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Authors:
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S Asano; M Seishima; Y Kitajima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental dermatology Volume: 26 ISSN: 0307-6938 ISO Abbreviation: Clin. Exp. Dermatol. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-06-25 Completed Date: 2001-07-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7606847 Medline TA: Clin Exp Dermatol Country: England |
Other Details:
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Languages: eng Pagination: 289-95 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Gifu University School of Medicine, Gifu, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acantholysis
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immunology* Humans Immunoglobulin G / immunology* Pemphigus / immunology* Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Receptors, Cell Surface / drug effects, metabolism* Receptors, Urokinase Plasminogen Activator Tumor Cells, Cultured Type C Phospholipases / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/PLAUR protein, human; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.11/Phosphoinositide Phospholipase C; EC 4.6.1.13/Phosphatidylinositol Diacylglycerol-Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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