| Phosphatidylinositol 4,5-bisphosphate is selectively retained by platelet-fibrin clots formed by thrombin. | |
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MedLine Citation:
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PMID: 2822029 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stimulation of human or rabbit platelets with thrombin in the presence of fibrinogen caused a large decrease, compared with unstimulated controls, in the amount of phosphatidylinositol 4,5-bisphosphate (PIP2) that could be extracted with acidified chloroform/methanol (60% at 60 s). In contrast, stimulation in the absence of added fibrinogen increased the amount of PIP2. The decrease was specific for PIP2, because similar decreases could not be demonstrated for other phosphoinositides or phospholipids. The interaction of polymerizing fibrin with stimulated platelets was required for the decrease in PIP2, since polymerized fibrin formed by reptilase did not cause the decrease in the amount of extractable PIP2, and inhibition by glycyl-L-prolyl-L-arginyl-L-proline of polymerization of fibrin formed by the action of thrombin prevented the large decrease in extractable PIP2. The decrease in extractable PIP2 could not be explained by increased degradation of PIP2, since sufficient degradation products were not formed. Thus, when platelets are stimulated with thrombin in the presence of fibrinogen, an association of polymerizing fibrin with the stimulated platelets occurs that leads to decreased extractability of PIP2. This may mean that PIP2 forms a specific association with platelet proteins that are involved in clot retraction. |
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Authors:
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J D Vickers; R L Kinlough-Rathbone; J F Mustard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 245 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1987-11-18 Completed Date: 1987-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 649-53 Citation Subset: IM |
Affiliation:
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Department of Pathology, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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pharmacology Animals Blood Coagulation* / drug effects Blood Platelets / drug effects, metabolism* Fibrin / metabolism* Fibrinogen / pharmacology Humans Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols / blood* Phospholipids / blood Rabbits Thrombin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylinositol 4,5-Diphosphate; 0/Phosphatidylinositols; 0/Phospholipids; 58-64-0/Adenosine Diphosphate; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen; EC 3.4.21.5/Thrombin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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