| Phosphatidylinositol 4-phosphate accumulates extracellularly upon xylanase treatment in tomato cell suspensions. | |
| | |
MedLine Citation:
|
PMID: 18419735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Various phosphoinositides have been implicated in plant defence signalling. Until now, such molecules have been exclusively related to intracellular signalling. Here, evidence is provided for the detection of extracellular phosphatidylinositol 4-phosphate (PI4P) in tomato cell suspensions. We have analysed and compared the intracellular and extracellular phospholipid profiles of [(32)P(i)]-prelabelled tomato cells, challenged with the fungal elicitor xylanase. These phospholipid patterns were found to be different, being phosphatidylinositol phosphate (PIP) the most abundant phospholipid in the extracellular medium. Moreover, while cells responded with a typical increase in phosphatidic acid and a decrease in intracellular PIP upon xylanase treatment, extracellular PIP level increased in a time- and dose-dependent manner. Using two experimental approaches, the extracellular PIP isoform was identified as PI4P. Addition of PI4P to tomato cell suspensions triggered the same defence responses as those induced by xylanase treatment. These include production of reactive oxygen species, accumulation of defence-related gene transcripts and induction of cell death. We demonstrate that extracellular PI4P is accumulated in xylanase-elicited cells and that exogenous application of PI4P mimics xylanase effects, suggesting its putative role as an intercellular signalling molecule. |
| | |
Authors:
|
Gabriela Gonorazky; Ana M Laxalt; Christa Testerink; Teun Munnik; Laura de la Canal |
Related Documents
:
|
346585 - Ergosterol biosynthesis in yeast. pathways in the late stages and their variation under... 16351325 - 5,10,15,20-tetrakis(n-methyl-4-pyridyl)-21h,23h-porphine (tmpyp) as a sensitizer for si... 6202345 - Control of oxygen affinity of hemoglobin in k562 cells induced by hemin. 19553565 - Effect of resveratrol and zinc on intracellular zinc status in normal human prostate ep... 20441885 - A rotating microfluidic array chip for staining assays. 21841785 - Repression of the genome organizer satb1 in regulatory t cells is required for suppress... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-17 |
Journal Detail:
|
Title: Plant, cell & environment Volume: 31 ISSN: 1365-3040 ISO Abbreviation: Plant Cell Environ. Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-08-15 Completed Date: 2008-10-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9309004 Medline TA: Plant Cell Environ Country: United States |
Other Details:
|
Languages: eng Pagination: 1051-62 Citation Subset: IM |
Affiliation:
|
Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata CC (1245), CP (7600) Mar del Plata, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Death
/
drug effects Dose-Response Relationship, Drug Endo-1,4-beta Xylanases / pharmacology* Extracellular Space / drug effects, metabolism* Gene Expression Regulation, Plant / drug effects Lycopersicon esculentum / cytology*, drug effects*, genetics, immunology Phosphatidylinositol Phosphates / metabolism*, pharmacology Plant Proteins / genetics, metabolism Respiratory Burst / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Phosphatidylinositol Phosphates; 0/Plant Proteins; 0/phosphatidylinositol 4-phosphate; EC 3.2.1.8/Endo-1,4-beta Xylanases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overexpression of Arabidopsis thaliana tryptophan synthase beta 1 (AtTSB1) in Arabidopsis and tomato...
Next Document: NaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified b...