Document Detail


Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis.
MedLine Citation:
PMID:  22321832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lipid kinase phosphatidylinositol 4-phosphate 5-kinase (PIP5K) produces a versatile signaling phospholipid, phosphatidylinositol 4,5-bisphosphate. Three PIP5K isozymes, PIP5K1A, PIP5K1B, and PIP5K1C, have been identified in mammals so far. Although the functions of these three PIP5K isozymes have been extensively studied in vitro, the in vivo physiological roles of these PIP5K isozymes remain largely unknown. In this study, we examined the functions of PIP5K1A and PIP5K1B in spermatogenesis, using Pip5k1a-knockout (KO), Pip5k1b-KO, and Pip5k1a/Pip5k1b double (D)-KO mice. Pip5k1a-KO and D-KO males were subfertile and completely sterile, respectively. F-actin in the seminiferous epithelium was disorganized in the D-KO mice, although F-actin bundles at the apical ectoplasmic specialization was not affected. D-KO seminiferous tubules contained a greatly decreased number of elongated spermatids. Flagella of sperm from Pip5k1a-KO and D-KO mice remarkably underwent morphological change, whereas Pip5k1b-KO sperm were morphologically normal. Notably, the flagellar shape of D-KO sperm was more severely impaired than that of Pip5k1a-KO sperm. These results suggest that PIP5K1A and PIP5K1B may coordinately and/or redundantly function in the maintenance of sperm number and morphology during spermatogenesis.
Authors:
Hiroshi Hasegawa; Junko Noguchi; Misuzu Yamashita; Risa Okada; Rika Sugimoto; Momoko Furuya; Takamitsu Unoki; Yuji Funakoshi; Tadashi Baba; Yasunori Kanaho
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-03
Journal Detail:
Title:  Biology of reproduction     Volume:  86     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-04     Completed Date:  2012-09-17     Revised Date:  2012-12-06    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  136, 1-12     Citation Subset:  IM    
Affiliation:
Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
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MeSH Terms
Descriptor/Qualifier:
Actins / physiology
Animals
Isoenzymes / genetics,  metabolism
Male
Mice
Mice, Knockout
Phosphotransferases (Alcohol Group Acceptor) / genetics,  metabolism*
Seminiferous Epithelium / metabolism
Seminiferous Tubules / metabolism
Sperm Tail / metabolism
Spermatids / metabolism
Spermatogenesis / physiology*
Chemical
Reg. No./Substance:
0/Actins; 0/Isoenzymes; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.68/1-phosphatidylinositol-4-phosphate 5-kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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