| Phosphatidylinositol 3-kinase activation attenuates the TLR2-mediated macrophage proinflammatory cytokine response to Francisella tularensis live vaccine strain. | |
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MedLine Citation:
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PMID: 21098227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An inadequate innate immune response appears to contribute to the virulence of Francisella tularensis following pulmonary infection. Studies in mice suggest that this poor response results from suppression of proinflammatory cytokine production early during infection, but the mechanisms involved are not understood. PI3K is known to regulate proinflammatory cytokine expression, but its exact role (positive versus negative) is controversial. We sought to clarify the role of PI3K in regulating proinflammatory signaling and cytokine production during infection with F. tularensis live vaccine strain (LVS). In this study, we demonstrate that the induction of TNF and IL-6 expression by LVS in mouse bone marrow-derived macrophages was markedly enhanced when PI3K activity was inhibited by either of the well-known chemical inhibitors, wortmannin or LY294002. The enhanced cytokine expression was accompanied by enhanced activation of p38 MAPK and ERK1/2, both of which were critical for LVS-induced expression of TNF and IL-6. LVS-induced MAPK activation and cytokine production were TLR2- and MyD88- dependent. PI3K/Akt activation was MyD88-dependent, but was surprisingly TLR2-independent. LVS infection also rapidly induced MAPK phosphatase-1 (MKP-1) expression; PI3K and TLR2 signaling were required. Peak levels of MKP-1 correlated closely with the decline in p38 MAPK and ERK1/2 phosphorylation. These data suggest that infection by LVS restrains the TLR2-triggered proinflammatory response via parallel activation of PI3K, leading to enhanced MKP-1 expression, accelerated deactivation of MAPKs, and suppression of proinflammatory cytokine production. This TLR2-independent inhibitory pathway may be an important mechanism by which Francisella suppresses the host's innate immune response. |
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Authors:
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Edward A Medina; Ian R Morris; Michael T Berton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-22 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 7562-72 Citation Subset: AIM; IM |
Affiliation:
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Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Androstadienes
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pharmacology Animals Bacterial Vaccines / genetics, immunology*, metabolism Bone Marrow Cells / immunology, metabolism Chromones / pharmacology Dual Specificity Phosphatase 1 / genetics, immunology, metabolism Enzyme Inhibitors / pharmacology Francisella tularensis / immunology*, metabolism Immunity, Innate / drug effects, genetics, immunology* Inflammation / genetics, immunology, metabolism Interleukin-6 / genetics, immunology, metabolism Macrophages / immunology, metabolism Mice Mice, Mutant Strains Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors, genetics, immunology, metabolism Morpholines / pharmacology Myeloid Differentiation Factor 88 / genetics, immunology, metabolism Phosphatidylinositol 3-Kinases / antagonists & inhibitors, genetics, immunology*, metabolism Phosphorylation / drug effects, genetics, immunology Proto-Oncogene Proteins c-akt / genetics, immunology, metabolism Signal Transduction / drug effects, genetics, immunology* Toll-Like Receptor 2 / genetics, immunology*, metabolism Tularemia / genetics, immunology*, metabolism, prevention & control Tumor Necrosis Factor-alpha / genetics, immunology, metabolism Vaccines, Attenuated / genetics, immunology, metabolism p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors, genetics, immunology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 AI057986/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Androstadienes; 0/Bacterial Vaccines; 0/Chromones; 0/Enzyme Inhibitors; 0/Interleukin-6; 0/Morpholines; 0/Myd88 protein, mouse; 0/Myeloid Differentiation Factor 88; 0/Tlr2 protein, mouse; 0/Toll-Like Receptor 2; 0/Tumor Necrosis Factor-alpha; 0/Vaccines, Attenuated; 154447-36-6/2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; 19545-26-7/wortmannin; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.1.3.48/Dual Specificity Phosphatase 1; EC 3.1.3.48/Dusp1 protein, mouse |
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