Document Detail


Phosphatidylethanolamine synthesis is required for optimal virulence of Brucella abortus.
MedLine Citation:
PMID:  18931122     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Brucella cell envelope contains the zwitterionic phospholipids phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Synthesis of PC occurs exclusively via the PC synthase pathway, implying that the pathogen depends on the choline synthesized by the host cell to form PC. Notably, PC is necessary to sustain a chronic infection process, which suggests that the membrane lipid content is relevant for Brucella virulence. In this study we investigated the first step of PE biosynthesis in B. abortus, which is catalyzed by phosphatidylserine synthase (PssA). Disruption of pssA abrogated the synthesis of PE without affecting the growth in rich complex medium. In minimal medium, however, the mutant required choline supplementation for growth, suggesting that at least PE or PC is necessary for Brucella viability. The absence of PE altered cell surface properties, but most importantly, it impaired several virulence traits of B. abortus, such as intracellular survival in both macrophages and HeLa cells, the maturation of the replicative Brucella-containing vacuole, and mouse colonization. These results suggest that membrane phospholipid composition is critical for the interaction of B. abortus with the host cell.
Authors:
Lucas Bukata; Silvia Altabe; Diego de Mendoza; Rodolfo A Ugalde; Diego J Comerci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-17
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-26     Completed Date:  2008-12-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8197-203     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, CONICET, San Martín, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics,  metabolism
Brucella abortus / genetics,  metabolism*,  pathogenicity*
Brucellosis / microbiology
CDPdiacylglycerol-Serine O-Phosphatidyltransferase / genetics,  metabolism
DNA, Bacterial / genetics
Female
Gene Knockout Techniques
Genes, Bacterial
Hela Cells
Humans
Macrophages / microbiology
Mice
Mice, Inbred BALB C
Mutation
Phosphatidylcholines / biosynthesis
Phosphatidylethanolamines / biosynthesis*
Plasmids
Virulence
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 39382-08-6/phosphatidylethanolamine; EC 2.7.8.8/CDPdiacylglycerol-Serine O-Phosphatidyltransferase
Comments/Corrections

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