Document Detail


Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants.
MedLine Citation:
PMID:  17979985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function of phosphatidylcholine (PC) in the bacterial cell envelope remains cryptic. We show here that productive interaction of the respiratory pathogen Legionella pneumophila with host cells requires bacterial PC. Synthesis of the lipid in L. pneumophila was shown to occur via either phospholipid N-methyltransferase (PmtA) or phosphatidylcholine synthase (PcsA), but the latter pathway was demonstrated to be of predominant importance. Loss of PC from the cell envelope caused lowered yields of L. pneumophila within macrophages as well as loss of high multiplicity cytotoxicity, while mutants defective in PC synthesis could be complemented either by reintroduction of PcsA or by overproduction of PmtA. The lowered yields and reduced cytotoxicity in mutants with defective PC biosynthesis were due to three related defects. First, there was a poorly functioning Dot/Icm apparatus, which delivers substrates required for intracellular growth into the cytosol of infected cells. Second, there was reduced bacterial binding to macrophages, possibly due to loss of PC or a PC derivative on the bacterium that is recognized by the host cell. Finally, strains lacking PC had low steady-state levels of flagellin protein, a deficit that had been previously associated with the phenotypes of lowered cytotoxicity and poor cellular adhesion.
Authors:
Gloria M Conover; Fernando Martinez-Morales; Matthew I Heidtman; Zhao-Qing Luo; May Tang; Cui Chen; Otto Geiger; Ralph R Isberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-11-02
Journal Detail:
Title:  Cellular microbiology     Volume:  10     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-03-07     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  514-28     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / genetics
Bacterial Proteins / genetics,  physiology
Flagellin / metabolism
Legionella pneumophila / growth & development,  metabolism,  pathogenicity*
Macrophages / microbiology
Phosphatidyl-N-Methylethanolamine N-Methyltransferase / genetics,  physiology
Phosphatidylcholines / biosynthesis*,  physiology
Transferases (Other Substituted Phosphate Groups) / genetics,  physiology
Virulence
Virulence Factors / physiology*
Grant Support
ID/Acronym/Agency:
P30 DK34928/DK/NIDDK NIH HHS; R37 AI023538/AI/NIAID NIH HHS; R37 AI023538-22/AI/NIAID NIH HHS; T32AI1007422/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Phosphatidylcholines; 0/Virulence Factors; 12777-81-0/Flagellin; EC 2.1.1.71/Phosphatidyl-N-Methylethanolamine N-Methyltransferase; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 2.7.8.24/CDP-diacylglycerol choline O-phosphatidyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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