| Phosphatidylcholine synthesis in the rat: the substrate for methylation and regulation by choline. | |
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MedLine Citation:
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PMID: 2333015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two lines of evidence led us to reexamine the possibility that methylation of phosphoethanolamine and its partially methylated derivatives, in addition to methylation of the corresponding phosphatidyl derivatives, plays a role in mammalian phosphatidylcholine biosynthesis: (a) Results obtained by Salerno and Beeler with rat [Salerno, D.M. and Beeler, D.A. (1973) Biochim. Biophys. Acta 326, 325-338] appear to strongly support such a role for methylation of phosphobases; (b) Such reactions have recently been shown to play major roles in phosphatidylcholine synthesis by higher plants [see Datko, A.H. and Mudd, S.H. (1988) Plant Physiol. 88, 854-861 and references therein]. We found that, following continuous labeling of rat liver with L-[methyl-3H]methionine for 10.4 min (intraperitoneal administration) or for 0.75 min (intraportal administration), virtually no 3H was detected in methylated derivatives of phosphoethanolamine, but readily detectable amounts of 3H were present in the base moiety of each methylated derivative of phosphatidylethanolamine. Thus, there was no indication that phospho-base methylation makes a significant contribution. Studies of cultured rat hepatoma cells showed definitively for the first time in a mammalian system that choline deprivation up-regulates the rate of flow of methyl groups originating in methionine into phosphatidylethanolamine and derivatives. Even under these conditions, methylation of phosphoethanolamine bases appeared to play a negligible role. |
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Authors:
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A H Datko; R R Aksamit; S H Mudd |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Lipids Volume: 25 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-06-06 Completed Date: 1990-06-06 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 135-42 Citation Subset: IM |
Affiliation:
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Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda, MD 20892. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Choline / metabolism, pharmacology* Ethanolamines / metabolism Liver / drug effects, metabolism* Liver Neoplasms, Experimental / metabolism Male Methionine / analogs & derivatives, metabolism Methylation Phosphatidylcholines / biosynthesis* Rats Rats, Inbred Strains Tritium Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Ethanolamines; 0/Phosphatidylcholines; 10028-17-8/Tritium; 10332-17-9/methionine methyl ester; 1071-23-4/phosphorylethanolamine; 62-49-7/Choline; 63-68-3/Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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