| Phosphatidylcholine catabolism in the MCF-7 cell cycle. | |
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MedLine Citation:
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PMID: 17167537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The catabolism of phosphatidylcholine (PtdCho) appears to play a key role in regulating the net accumulation of the lipid in the cell cycle. Current protocols for measuring the degradation of PtdCho at specific cell-cycle phases require prolonged periods of incubation with radiolabelled choline. To measure the degradation of PtdCho at the S and G2 phases in the MCF-7 cell cycle, protocols were developed with radiolabelled lysophosphatidylcholine (lysoPtdCho), which reduces the labelling period and minimizes the recycling of labelled components. Although most of the incubated lysoPtdCho was hydrolyzed to glycerophosphocholine (GroPCho) in the medium, the kinetics of the incorporation of label into PtdCho suggests that the labelled GroPCho did not contribute significantly to cellular PtdCho formation. A protocol which involved exposing the cells twice to hydroxyurea, was also developed to produce highly synchronized MCF-7 cells with a profile of G1:S:G2/M of 90:5:5. An analysis of PtdCho catabolism in the synchronized cells following labelling with lysoPtdCho revealed that there was increased degradation of PtdCho in early to mid-S phase, which was attenuated in the G2/M phase. The results suggest that the net accumulation of PtdCho in MCF-7 cells may occur in the G2 phase of the cell cycle. |
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Authors:
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Weiyang Lin; Gilbert Arthur |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 84 ISSN: 0829-8211 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-12-14 Completed Date: 2007-04-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: Canada |
Other Details:
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Languages: eng Pagination: 737-44 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, Winnipeg, MB R3E 0W3, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle* Cell Proliferation G2 Phase Glycerylphosphorylcholine / metabolism Humans Lysophosphatidylcholines / metabolism Metabolism Phosphatidylcholines / metabolism* Radioactive Tracers S Phase Tritium / analysis Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Radioactive Tracers; 10028-17-8/Tritium; 563-24-6/Glycerylphosphorylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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