Document Detail

Phosphatidylcholine catabolism in the MCF-7 cell cycle.
MedLine Citation:
PMID:  17167537     Owner:  NLM     Status:  MEDLINE    
The catabolism of phosphatidylcholine (PtdCho) appears to play a key role in regulating the net accumulation of the lipid in the cell cycle. Current protocols for measuring the degradation of PtdCho at specific cell-cycle phases require prolonged periods of incubation with radiolabelled choline. To measure the degradation of PtdCho at the S and G2 phases in the MCF-7 cell cycle, protocols were developed with radiolabelled lysophosphatidylcholine (lysoPtdCho), which reduces the labelling period and minimizes the recycling of labelled components. Although most of the incubated lysoPtdCho was hydrolyzed to glycerophosphocholine (GroPCho) in the medium, the kinetics of the incorporation of label into PtdCho suggests that the labelled GroPCho did not contribute significantly to cellular PtdCho formation. A protocol which involved exposing the cells twice to hydroxyurea, was also developed to produce highly synchronized MCF-7 cells with a profile of G1:S:G2/M of 90:5:5. An analysis of PtdCho catabolism in the synchronized cells following labelling with lysoPtdCho revealed that there was increased degradation of PtdCho in early to mid-S phase, which was attenuated in the G2/M phase. The results suggest that the net accumulation of PtdCho in MCF-7 cells may occur in the G2 phase of the cell cycle.
Weiyang Lin; Gilbert Arthur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  84     ISSN:  0829-8211     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-12-14     Completed Date:  2007-04-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  737-44     Citation Subset:  IM    
Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, Winnipeg, MB R3E 0W3, Canada.
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MeSH Terms
Cell Cycle*
Cell Proliferation
G2 Phase
Glycerylphosphorylcholine / metabolism
Lysophosphatidylcholines / metabolism
Phosphatidylcholines / metabolism*
Radioactive Tracers
S Phase
Tritium / analysis
Tumor Cells, Cultured
Reg. No./Substance:
0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Radioactive Tracers; 10028-17-8/Tritium; 563-24-6/Glycerylphosphorylcholine

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