Document Detail

Phosphatidylcholine biosynthesis in the neuroblastoma-glioma hybrid cell line NG108-15: stimulation by phorbol esters.
MedLine Citation:
PMID:  3772385     Owner:  NLM     Status:  MEDLINE    
We have examined the effects of phorbol esters on phosphatidylcholine (PtdCho) metabolism in the neuroblastoma-glioma hybrid cell line NG108-15. 12-O-Tetradecanoylphorbol-13-acetate (TPA), 100 nM, stimulated twofold the incorporation of [3H]choline into PtdCho during 2 h of incubation at 37 degrees C. This effect of TPA was concentration dependent, exhibiting an EC50 of 24.5 +/- 4.4 nM. The effect of TPA was also time dependent and became apparent only after a lag period of 15-30 min. TPA also decreased the incorporation of [3H]choline into water-soluble cellular constituents in a manner whose concentration and time-dependence paralleled the changes observed in PtdCho content. HPLC analysis of this pool revealed that the levels of its major (85-95%) constituent, [3H]phosphocholine, were decreased by 29 +/- 5%, whereas those of [3H]glycerophosphocholine (0.5-2% of the pool) were increased by 84 +/- 4%. PtdCho labeling was also stimulated when cells were pulse labeled with [3H]choline and chased in the presence of TPA. The incorporation of [3H]inositol, [14C]ethanolamine, or [14C]serine into phospholipids was not affected by TPA. The non-tumor-promoting compounds phorbol and 4 alpha-phorbol-12,13-didecanoate (at 100 nM) were completely ineffective in modulating choline incorporation, whereas the biologically active analogs 4 beta-phorbol-12,13-didecanoate and 4 beta-phorbol-12,13-dibutyrate were as effective as TPA. We conclude that tumor-promoting phorbol esters can modulate PtdCho metabolism in neural-derived cells. The mechanisms mediating this effect and the possible involvement of PtdCho metabolism in normal signal transduction events and in the biological actions of tumor promoters are discussed.
M Liscovitch; A Freese; J K Blusztajn; R J Wurtman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  47     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1986-12-17     Completed Date:  1986-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1936-41     Citation Subset:  IM    
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MeSH Terms
Cell Line
Choline / metabolism
Ethanolamines / metabolism
Glioma / metabolism*
Inositol / metabolism
Neuroblastoma / metabolism*
Phorbol Esters / pharmacology*
Phosphatidylcholines / biosynthesis*
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Ethanolamines; 0/Phorbol Esters; 0/Phosphatidylcholines; 141-43-5/Ethanolamine; 16561-29-8/Tetradecanoylphorbol Acetate; 62-49-7/Choline; 6917-35-7/Inositol

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