Document Detail


Phosphatidic acid synthesis in mitochondria. Topography of formation and transmembrane migration.
MedLine Citation:
PMID:  10514455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The topography of formation and migration of phosphatidic acid (PA) in the transverse plane of rat liver mitochondrial outer membrane (MOM) were investigated. Isolated mitochondria and microsomes, incubated with sn-glycerol 3-phosphate and an immobilized substrate palmitoyl-CoA-agarose, synthesized both lyso-PA and PA. The mitochondrial and microsomal acylation of glycerophosphate with palmitoyl-CoA-agarose was 80-100% of the values obtained in the presence of free palmitoyl-CoA. In another series of experiments, both free polymyxin B and polymyxin B-agarose stimulated mitochondrial glycerophosphate acyltransferase activity approximately 2-fold. When PA loaded mitochondria were treated with liver fatty acid binding protein, a fifth of the phospholipid left the mitochondria. The amount of exportable PA reduced with the increase in the time of incubation. In another approach, PA-loaded mitochondria were treated with phospholipase A(2). The amount of phospholipase A(2)-sensitive PA reduced when the incubation time was increased. Taken together, the results suggest that lysophosphatidic acid (LPA) and PA are synthesized on the outer surface of the MOM and that PA moves to the inner membrane presumably for cardiolipin formation.
Authors:
T R Chakraborty; A Vancura; V S Balija; D Haldar
Related Documents :
5420955 - Turnover of the glycerolipids of pumpkin leaves. the importence of phosphatidylcholine.
14583095 - Structural elucidation of novel phosphocholine-containing glycosylinositol-phosphoceram...
11692115 - Occupational allergy to bumblebees: allergens of bombus terrestris.
4079915 - The partition of charged liposomes in aqueous two-phase systems.
1317035 - Inhibition of lipid peroxidation by prostaglandin oligomeric derivatives.
23179615 - Engineering e. coli for caffeic acid biosynthesis from renewable sugars.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29786-90     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, St. John's University, Jamaica, New York 11439, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acylation
Animals
Biological Transport
Carrier Proteins / metabolism
Cell Membrane / metabolism
Fatty Acid-Binding Proteins
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Male
Microsomes, Liver / enzymology
Mitochondria, Liver / metabolism*
Myelin P2 Protein / metabolism
Neoplasm Proteins*
Nerve Tissue Proteins*
Phosphatidic Acids / biosynthesis*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
GM-57643/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Myelin P2 Protein; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Phosphatidic Acids; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differential effect of retinoic acid on growth regulation by phorbol ester in human cancer cell line...
Next Document:  Differential phosphorylation paradigms dictate desensitization and internalization of the N-formyl p...