Document Detail

Phosphatidic acid synthesis in mitochondria. Topography of formation and transmembrane migration.
MedLine Citation:
PMID:  10514455     Owner:  NLM     Status:  MEDLINE    
The topography of formation and migration of phosphatidic acid (PA) in the transverse plane of rat liver mitochondrial outer membrane (MOM) were investigated. Isolated mitochondria and microsomes, incubated with sn-glycerol 3-phosphate and an immobilized substrate palmitoyl-CoA-agarose, synthesized both lyso-PA and PA. The mitochondrial and microsomal acylation of glycerophosphate with palmitoyl-CoA-agarose was 80-100% of the values obtained in the presence of free palmitoyl-CoA. In another series of experiments, both free polymyxin B and polymyxin B-agarose stimulated mitochondrial glycerophosphate acyltransferase activity approximately 2-fold. When PA loaded mitochondria were treated with liver fatty acid binding protein, a fifth of the phospholipid left the mitochondria. The amount of exportable PA reduced with the increase in the time of incubation. In another approach, PA-loaded mitochondria were treated with phospholipase A(2). The amount of phospholipase A(2)-sensitive PA reduced when the incubation time was increased. Taken together, the results suggest that lysophosphatidic acid (LPA) and PA are synthesized on the outer surface of the MOM and that PA moves to the inner membrane presumably for cardiolipin formation.
T R Chakraborty; A Vancura; V S Balija; D Haldar
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29786-90     Citation Subset:  IM    
Department of Biological Sciences, St. John's University, Jamaica, New York 11439, USA.
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MeSH Terms
Biological Transport
Carrier Proteins / metabolism
Cell Membrane / metabolism
Fatty Acid-Binding Proteins
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Microsomes, Liver / enzymology
Mitochondria, Liver / metabolism*
Myelin P2 Protein / metabolism
Neoplasm Proteins*
Nerve Tissue Proteins*
Phosphatidic Acids / biosynthesis*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Myelin P2 Protein; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Phosphatidic Acids; EC O-Acyltransferase

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