| Phosphatidic acid production, required for cholecystokinin octapeptide-stimulated amylase secretion from pancreatic acinar AR42J cells, is regulated by a wortmannin-sensitive process. | |
| | |
MedLine Citation:
|
PMID: 12821133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To investigate the role of phospholipids in exocytotic secretory events, we utilized rat pancreatic acinar AR42J cells that secreted amylase in response to cholecystokinin octapeptide (CCK-8). Wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K), was found to inhibit the secretion in a dose-dependent manner. When changes in cell membrane phospholipids were investigated before and after CCK-8 stimulation using [32P]orthophosphoric acid-labeled AR42J cells, we observed a rapid increase in phosphatidic acid (PtdOH) levels right after stimulation, which was not observed in non-stimulated cells. The increase, however, was suppressed by wortmannin pre-treatment, which also inhibited amylase secretion. Changes in other major phospholipids were not significant. These results indicate that CCK-8 induces amylase secretion through PI3K-regulated production of PtdOH in cell membranes. |
| | |
Authors:
|
Yoshiki Ikeda; Shin-ichi Fukuoka |
Related Documents
:
|
16316423 - Elevated serum levels of soluble e-cadherin in patients with primary sj?gren's syndrome. 9067633 - Differential expression of alpha 2-adrenoceptor subtypes in purified rat pancreatic isl... 16316423 - Elevated serum levels of soluble e-cadherin in patients with primary sj?gren's syndrome. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 306 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-06-24 Completed Date: 2003-08-11 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 943-7 Citation Subset: IM |
Affiliation:
|
Department of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Phosphatidylinositol 3-Kinase
/
metabolism Amylases / secretion* Androstadienes / pharmacology Animals Cell Membrane / metabolism Cholecystokinin / chemistry* Chromatography, Thin Layer Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Pancreas / cytology Phosphatidic Acids / metabolism* Phospholipids / chemistry Rats Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Androstadienes; 0/Enzyme Inhibitors; 0/Phosphatidic Acids; 0/Phospholipids; 19545-26-7/wortmannin; 9011-97-6/Cholecystokinin; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of GTP cyclohydrolase I by alternative splicing in mononuclear cells.
Next Document: Secondary structures in CpG oligonucleotides affect immunostimulatory activity.