Document Detail


Phosphatidic acid formation is required for extracellular ATP-mediated nitric oxide production in suspension-cultured tomato cells.
MedLine Citation:
PMID:  20356346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
*In animals and plants, extracellular ATP exerts its effects by regulating the second messengers Ca(2+), nitric oxide (NO) and reactive oxygen species (ROS). In animals, phospholipid-derived molecules, such as diacylglycerol, phosphatidic acid (PA) and inositol phosphates, have been associated with the extracellular ATP signaling pathway. The involvement of phospholipids in extracellular ATP signaling in plants, as it is established in animals, is unknown. *In vivo phospholipid signaling upon extracellular ATP treatment was studied in (32)P(i)-labeled suspension-cultured tomato (Solanum lycopersicum) cells. *Here, we report that, in suspension-cultured tomato cells, extracellular ATP induces the formation of the signaling lipid phosphatidic acid. Exogenous ATP at doses of 0.1 and 1 mM induce the formation of phosphatidic acid within minutes. Studies on the enzymatic sources of phosphatidic acid revealed the participation of both phospholipase D and C in concerted action with diacylglycerol kinase. *Our results suggest that extracellular ATP-mediated nitric oxide production is downstream of phospholipase C/diacylglycerol kinase activation.
Authors:
Daniela J Sueldo; Noelia P Foresi; Claudia A Casalongu?; Lorenzo Lamattina; Ana M Laxalt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The New phytologist     Volume:  185     ISSN:  1469-8137     ISO Abbreviation:  New Phytol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-01     Completed Date:  2010-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  England    
Other Details:
Languages:  eng     Pagination:  909-16     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biol?gicas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology*
Calcium / metabolism
Cells, Cultured
Diacylglycerol Kinase / metabolism
Enzyme Activation / drug effects
Extracellular Space / drug effects,  metabolism*
Lycopersicon esculentum / cytology*,  drug effects,  enzymology,  metabolism*
Nitric Oxide / biosynthesis*
Phosphatidic Acids / biosynthesis*
Phospholipase D / metabolism
Suspensions
Type C Phospholipases / metabolism
Chemical
Reg. No./Substance:
0/Phosphatidic Acids; 0/Suspensions; 10102-43-9/Nitric Oxide; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium; EC 2.7.1.107/Diacylglycerol Kinase; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.4/Phospholipase D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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