Document Detail

Association of phosphatidic acid with the bovine mitochondrial ADP/ATP carrier.
MedLine Citation:
PMID:  19902971     Owner:  NLM     Status:  MEDLINE    
The beef heart adenine nucleotide carrier protein (Anc) of the inner mitochondrial membrane can be purified in a form stabilized by binding the inhibitor carboxyatractyloside. The protein is copurified with bound lipid. We show for the first time that phosphatidic acid, although a minor component, is one of the lipids bound to Anc. The short spin-lattice relaxation time found by (31)P magic angle spinning nuclear magnetic resonance (MAS/NMR) for phosphatidic acid indicates that it is tightly bound to the protein. However, this lipid also has a comparatively small chemical shift anisotropy, suggesting that it can undergo rapid reorientation in space. In contrast, most of the lipid bound to Anc shows anisotropic motion typical of a bilayer arrangement. The phosphatidic acid that is detected in the purified preparation of Anc is also shown to be present initially in the unfractionated mitochondria, prior to the isolation of Anc. In Triton-solubilized mitochondria, phosphatidic acid, cardiolipin, phosphatidylethanolamine, and phosphatidylcholine exhibit resonance lines in the static (31)P NMR spectra, but in the purified Anc, only the phosphatidylethanolamine and phosphatidylcholine can be detected by this method, even though the other lipids are still present. This demonstrates that the phosphatidic acid and cardiolipin are interacting with the Anc. The thermal denaturation of the Anc was determined by differential scanning calorimetry. The protein denatures at 74 degrees C both before and after the NMR studies with the same characteristics.
Richard M Epand; Raquel F Epand; Bob Berno; Ludovic Pelosi; Gérard Brandolin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  48     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-22     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12358-64     Citation Subset:  IM    
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, OntarioL8N 3Z5, Canada.
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MeSH Terms
Cardiolipins / chemistry
Mitochondrial ADP, ATP Translocases / chemistry*,  isolation & purification
Molecular Conformation
Nuclear Magnetic Resonance, Biomolecular
Phosphatidic Acids / chemistry*
Protein Binding
Reg. No./Substance:
0/Cardiolipins; 0/Phosphatidic Acids; 9068-80-8/Mitochondrial ADP, ATP Translocases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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