Document Detail

Phosphate depletion enhances the stability of the amphotropic murine leukemia virus receptor mRNA.
MedLine Citation:
PMID:  9448695     Owner:  NLM     Status:  MEDLINE    
Through its specific receptor, the amphotropic murine leukemia virus (MLV) infects cells from many mammals, including humans. We have previously demonstrated that levels of human amphotropic MLV receptor (pit2) mRNA varied considerably in different human cell lines. Removal of phosphate from the culture medium led to increases in the amount of pit2 mRNA and the quantity of a 71-kDa protein specifically recognized by antibodies against Pit2. To determine if the increases in pit2 mRNA and protein levels were due to a transcriptional effect, the pit2 promoter region was cloned. This region was characterized and found to contain a functional TATA-less promoter that under our experimental conditions does not respond to phosphate depletion. Instead, pit2 mRNA was found to be more stable in response to Pi depletion. These results suggest that the increase in pit2 mRNA levels observed in response to Pi depletion occurs at a posttranscriptional level and is due to enhanced mRNA stability.
M L Chien; E O'Neill; J V Garcia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  240     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-02-19     Completed Date:  1998-02-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  109-17     Citation Subset:  IM    
Department of Virology & Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
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MeSH Terms
Base Sequence
Binding Sites
Carrier Proteins / biosynthesis*,  genetics*
Cell Line
Cloning, Molecular
Culture Media
In Situ Hybridization, Fluorescence
Leukemia Virus, Murine / physiology*
Membrane Glycoproteins*
Membrane Proteins / biosynthesis*,  genetics*
Molecular Sequence Data
Phosphates / metabolism*
Promoter Regions, Genetic
RNA, Messenger / metabolism*
Receptors, Virus / biosynthesis*
Recombinant Proteins / biosynthesis
Restriction Mapping
Transcription Factors
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/Culture Media; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Phosphates; 0/RNA, Messenger; 0/Receptors, Virus; 0/Recombinant Proteins; 0/Transcription Factors; 0/ecotropic murine leukemia virus receptor

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