| Phosphate-Induced Apoptosis in Human Peritoneal Mesothelial Cells in vitro. | |
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MedLine Citation:
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PMID: 21677430 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: It has been demonstrated that phosphate uptake through the type III sodium-dependent phosphate co-transporter, Pit-1, induced apoptosis of aortic vascular smooth muscle cells and endothelial cells in vitro. However, the apoptotic effects of high phosphate (HP) level in human peritoneal mesothelial cells (HPMCs) are not known. Methods: To examine whether Pit-1 is expressed in HPMCs, we checked the Western blot assay of immunoreactive Pit-1 and the transcription of Pit-1 by reverse transcriptase PCR. We treated several different phosphate concentrations (1-4 mM) and calcium concentrations (1.8 and 2.8 mM) on HPMCs to assess the effects of concentration. MTT, TUNEL assays, and flow cytometry analysis using Annexin V and propidium iodide were performed to identify cell death and apoptosis. Bax and Bcl-2 by Western blot and caspase-3 activity were evaluated by colorimetric assay. In addition, phosphonoformic acid (PFA) and pan-caspase inhibitor, Z-VAD-FMK, were given to prevent phosphate-induced apoptosis. Results: Pit-1 expression on HPMCs was demonstrated. Apoptosis in HPMCs significantly increased with a high concentration of phosphate in a dose- and time-dependent manner, and was enhanced in the presence of 2.8 mM calcium. HP concentrations significantly decreased the anti-apoptotic Bcl-2/Bax ratio and increased caspase-3 activity. The treatment with PFA and Z-VAD-FMK prevented cell death by HP. Conclusion: Phosphate uptake through Pit-1 induces apoptosis in HPMCs by a caspase-related mechanism. |
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Authors:
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Jong-Won Park; Ju-Min Yook; Hye-Myung Ryu; Soon-Youn Choi; Masayo Morishita; Jun-Young Do; Sun-Hee Park; Chan-Duck Kim; Ji-Young Choi; Ho-Young Chung; Yong-Lim Kim |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-15 |
Journal Detail:
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Title: American journal of nephrology Volume: 34 ISSN: 1421-9670 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8109361 Medline TA: Am J Nephrol Country: - |
Other Details:
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Languages: ENG Pagination: 77-86 Citation Subset: - |
Copyright Information:
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Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
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Department of Internal Medicine, Yeungnam University College of Medicine, Daegu, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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